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Method of sample analysis

  • US 5,158,868 A
  • Filed: 10/15/1990
  • Issued: 10/27/1992
  • Est. Priority Date: 07/17/1987
  • Status: Expired due to Fees
First Claim
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1. A method for measuring a constituent of interest of a biological fluid or the like comprising the steps ofproviding a reaction chamber that contains an immobilized enzyme capable of modifying a constituent of interest,providing a measuring system,placing a first portion of a biological fluid to be analyzed in said reaction chamber and concurrently exposing a second unmodified portion of said biological fluid to said measuring system to provide a first data output,oscillating said first biological fluid portion with bidirectional flow in said reaction chamber to facilitate modification by said immobilized enzyme of said constituent of interest in said biological fluid,then exposing said first portion of said biological fluid to be analyzed to said measuring system to provide a second data output, andmodifying said second data output as a function of said first data output to provide an indication of the actual amount of said constituent of interest in said biological fluid.

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