Process for preparing solid perfluorocarbon polymer supports having attached perfluorocarbon-substituted ligand or binder

• US 5,158,880 A
• Filed: 09/23/1988
• Issued: 10/27/1992
• Est. Priority Date: 09/23/1988
• Status: Expired due to Fees

• Current Assignee: Arkion Life Sciences
• Original Assignee: E. I. du Pont de Nemours and Company (Corteva, Inc.)
• Inventors: Eveleigh, John W. D.
• Primary Examiner(s): Moskowitz, Margaret
• Assistant Examiner(s): Marschel, Ardin H.
• Application Number:
  US07/248,386
• Time in Patent Office: 1,495 Days
• Field of Search: 204/59 F, 204/82, 427/322, 427/338, 435/6, 435/7, 435/181, 435/4, 435/7.1, 435/7.5, 435/7.6, 435/7.92, 435/180, 436/501, 436/532, 436/126, 530/387, 935/86, 935/110
• US Class Current: 435/180
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/321 consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: Y10T 436/196666 Carbon containing compound [e.g., vinylchloride, etc.]

Abstract

A process for preparing solid perfluorocarbon polymer supports permitting uniform and secure attachment of perfluorocarbon-substituted ligands or binders to carriers is provided utilizing pretreatment of the carriers with water miscible organic solvents.

Claims

1. A process for attaching a perfluorocarbon-substituted ligand or binder to a perfluorocarbon polymer carrier comprising the steps of:

(a) contacting a perfluorocarbon polymer carrier with a first neat water miscible organic solvent capable of wetting said carrier to produce a wetted carrier;

(b) separating said wetted carrier from said solvent;

(c) adding an aqueous solution of a second water miscible organic solvent to said wetted carrier wherein said solution is capable of wetting said carrier;

(d) adding a perfluorocarbon-substituted ligand or binder solution containing said second water miscible organic solvent to said wetted carrier;

provided that neither solution in steps (c) and (d) is at a denaturing concentration for said ligand or binder; and

(e) stirring the mixture of step (d) to allow for secure and uniform attachment of said ligand or binder to said wetted carrier.

2. The method of claim 1 wherein the first water miscible organic solvent is selected from the group consisting of alcohol, dioxane, acetonitrile, tetrahydrofuran and dimethylformamide.

3. The method claim 2 wherein the alcohol is methanol.

4. The method of claim 1 wherein the second water miscible organic solvent is selected from the group consisting of dioxane, acetonitrile, tetrahydrofuran and dimethylformamide.