Flow imaging cytometer

• US 5,159,397 A
• Filed: 09/05/1991
• Issued: 10/27/1992
• Est. Priority Date: 02/27/1991
• Status: Expired due to Term

• Current Assignee: Toa Medical Electronics Co., Ltd.
• Original Assignee: Toa Medical Electronics Co., Ltd.
• Inventors: Kosaka, Tokihiro, Ogino, Shinichi
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US07/755,304
• Time in Patent Office: 418 Days
• Field of Search: 356/23, 356/72, 356/73, 356/39, 356/318, 356/417, 250/461.2, 382/6
• US Class Current: 356/73
• CPC Class Codes: G01N 15/1427 with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/1413 Hydrodynamic focussing
• CPC Class Codes: G01N 2015/144 Imaging characterised by its optical setup

Abstract

An imaging flow cytometer is provided with a continuous-emission light source for continuously monitoring cells passing through the image capturing area of a video camera for cell-image capturing, and with an excitation light source for picking up a fluorescent image of a cell. When a line sensor monitoring cell passage through the cytometer senses such cell passage, the cell is irradiated with strobe light and then, after waiting for the cell to move a fixed distance, with the excitation light. Thus, a particle analyzer is provided in which an image by white light resulting from the strobe light and a fluorescent image resulting from the excitation light can be captured simultaneously by a single video camera in either upper and lower halves or right and left halves of one imaged frame.

Claims

1. A flow imaging cytometer comprising:

a flow cell formed to include a flat flow path for causing a specimen solution containing particle components to be sensed to flow as a flat stream;

first and third light sources arranged on a first side of said flow cell for irradiating the specimen solution in said flow cell with pulsed light;

first image capturing means arranged on an opposite side of said flow cell for capturing still pictures of the particle components in the specimen solution irradiated by said first and third light sources;

a second light source arranged on the first side of said flow cell for irradiating the specimen solution in said flow cell with light continuously;

second image capturing means arranged on the opposite side of said flow cell for picking up an image of the specimen solution irradiated by said second light source;

processing means for executing prescribed analysis based upon image data from said first and second image capturing means; and

light-source control means for detecting the particle components based upon the image data from said second image capturing means, and on the basis of such detection, for causing said third light source to emit light first, followed by said first light source upon passage of a prescribed time, within an image capturing period of said first image capturing means;

wherein said first light source is a light source for exciting fluorescence, said third light source is a light source for emitting white light, and the image resulting from said first light source and the image resulting from said third light source are each captured in a different area on a light-detecting surface of said first image capturing means.

2. The flow imaging cytometer according to claim 1, wherein said first image capturing means has a two-dimensional image capturing area on the flow of the specimen solution, said second image capturing means has a linear image capturing area on the flow of the specimen solution, the image capturing area of said second image capturing means is formed so as to cross the flow of the specimen solution within the image capturing area of said first image capturing means, the image capturing area of said first image capturing means is divided into a zone which includes, and a zone which does not include, the image capturing area of the second image capturing means, and an image in one of these zones resulting from irradiation by said third light source and an image in the other of these zones resulting from irradiation by said first light source are captured by said first image capturing means.

3. The flow imaging cytometer according to claim 2, further comprising masking means for masking light irradiating said first image capturing means in such a manner that the two images do not overlap each other on the light-detecting surface of said first image capturing means.

4. The flow imaging cytometer according to claim 2, further comprising means for forming the irradiating light from said first light source into an elongated elliptical shape.

5. The flow imaging cytometer according to any one of claims 1 through 4, wherein a light-detecting system of a fluorescent image is provided with an image intensifier, and said image intensifier is operated only when the fluorescent image is captured.