Flow cell mechanism in flow imaging cytometer

• US 5,159,403 A
• Filed: 03/19/1991
• Issued: 10/27/1992
• Est. Priority Date: 11/22/1990
• Status: Expired due to Fees

• Current Assignee: Toa Medical Electronics Co., Ltd.
• Original Assignee: Toa Medical Electronics Co., Ltd.
• Inventors: Kosaka, Tokihiro
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US07/671,531
• Time in Patent Office: 588 Days
• Field of Search: 356/73, 356/243, 250/201.2, 250/201.3, 250/201.7, 250/252.1
• US Class Current: 356/243.2
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/1452 Adjustment of focus; Alignment
• CPC Class Codes: G01N 2021/058 Flat flow cell

Abstract

A flow imaging cytomer for imaging the dimensions and shapes of particle components in a flow of a specimen solution has a slender filament serving as a focusing reference provided in a flat imaging zone through which the specimen solution flows in order for the particle components to be imaged. By imaging the filament-shaped focusing reference and adjusting position in accordance with the sharpness of an image of the reference, the particle components in the specimen solution can be brought into sharp focus.

Claims

1. In a flow imaging cytometer in which a specimen solution containing particle components such as cells is made to flow, while sheathed by a sheathing liquid, through a flat flow path within a flow cell, a still image of the specimen solution flow is photographed by light irradiating means and imaging means so arranged that an optic axis thereof intersects the flow path, and the still image is subjected to image processing, whereby analysis such as classification and enumeration of the particle components contained in the specimen solution is performed, a flow cell mechanism which includes:

a focusing reference for focus adjustment comprising an element suspended within the zone through which the specimen solution flows and is photographed.

2. The flow cell mechanism according to claim 1, wherein said focusing reference element is a filament-shaped body.

3. The flow cell mechanism according to claim 2, wherein said filament-shaped body is suspended in and extends longitudinally through said flat flow path and further extends through a sampling nozzle of the flow cell.

4. The flow cell mechanism according to claim 3, wherein said filament-shaped body has a diameter of 2-5 μ

m.

5. The flow cell mechanism according to claim 4, further comprising focal-point adjusting means for adjusting the relative position of said flow cell and said imaging system in correlation with sharpness of the image of said focusing reference when said focusing reference is imaged.

6. The flow cell mechanism according to claim 3, further comprising focal-point adjusting means for adjusting the relative position of said flow cell and said imaging system in correlation with sharpness of the image of said focusing reference when said focusing reference is imaged.

7. The flow cell mechanism according to claim 2, further comprising focal-point adjusting means for adjusting the relative position of said flow cell and said imaging system in correlation with sharpness of the image of said focusing reference when said focusing reference is imaged.

8. The flow cell mechanism according to claim 1, further comprising focal-point adjusting means for adjusting the relative position of said imaging means and said flow cell in correlation with sharpness of the image of said focusing reference when said focusing reference is imaged.

9. In flow imaging cytometry in which a specimen solution containing particle components such as cells is formed into a flat, laminar flow sheathed by a sheathing liquid, a still image of the specimen solution flow is photographed in a flow zone and the still image is subjected to image processing, whereby analysis such as classification and enumeration of the particle components contained in the specimen solution is performed, an automatic focal-point adjustment method comprising the steps of:

imaging a filament-shaped body as a focusing reference for focus adjustment provided in the flow zone; and

adjusting position in accordance with sharpness of an image of said filament-shaped body, thereby performing a focus adjustment with regard to the particle components in the specimen solution.

10. the focal-point adjustment method according to claim 9, further comprising a step of continuously imaging said filament-shaped body while making fine adjustments in relative positions of the flow cell and a lens system.

11. The focal-point adjustment method according to claim 10, further comprising the steps of:

continuously monitoring state of focus in accordance with said sharpness of the image, and

adjusting the relative position of said flow cell and said imaging system when the state of focus is indicative of a value less than a predetermined value.

12. The focal-point adjustment method according to claim 9, further comprising the steps of:

continuously monitoring the state of focus in accordance with said sharpness of the image; and

adjusting the relative position of said flow cell and said imaging system when the state of focus is indicative of a value less than a predetermined value.