Recombinant DNA vectors capable of expressing apoaequorin in E. coli

• US 5,162,227 A
• Filed: 03/17/1988
• Issued: 11/10/1992
• Est. Priority Date: 12/31/1984
• Status: Expired due to Term

• Current Assignee: The University of Georgia Research Foundation, Inc.
• Original Assignee: The University of Georgia Research Foundation, Inc.
• Inventors: Cormier, Milton J.
• Primary Examiner(s): Schwartz, Richard A.
• Application Number:
  US07/173,045
• Time in Patent Office: 1,699 Days
• Field of Search: 435/68, 435/70, 435/91, 435/172.1, 435/172.3, 435/243, 435/253, 435/317, 435/320, 435/252.33, 935/9-11, 935/22-23, 935/27, 935/41, 935/56, 935/55, 935/60, 935/66, 935/72, 536/27-29
• US Class Current: 435/252.33
• CPC Class Codes: C07K 14/43595 from coelenteratae, e.g. medusae

Abstract

A gene which codes for the protein apoaequorin is disclosed along with recombinant DNA vectors containing this gene.

Claims

1. An isolated DNA molecule, which comprises a nucleotide sequence coding for a peptide of the formula ##STR9##

2. The molecule of claim 1, wherein said sequence is ##STR10##

3. The molecule of claim 2 wherein said molecule further comprises a non-expressed segment 3'"'"' to said sequence and a non-expressed segment 5'"'"' to said sequence, wherein said molecule is ##STR11##

4. The molecule of claim 2, wherein said molecule is single-stranded DNA.

5. The molecule of claim 2, wherein said molecule is double-stranded DNA.

6. A recombinant DNA vector capable of reproducing in a microorganism, wherein said vector comprises a nucleotide sequence coding for a peptide of the formula ##STR12##

7. The vector of claim 6, wherein said sequence is ##STR13##

8. The vector of claim 6, wherein said sequence is preceded by a lac promotor.

9. A genetically engineered E. coli microorganism wherein said microorganism comprises the vector of claim 6.

10. A microorganism of claim 9, wherein said vector comprises a nucleotide sequence of the formula ##STR14##