Composition and method for producing superovulation in mammals

• US 5,162,306 A
• Filed: 03/08/1991
• Issued: 11/10/1992
• Est. Priority Date: 01/23/1987
• Status: Expired due to Fees

• Current Assignee: AUSA International Incorporated
• Original Assignee: Lloyd E. Donaldson
• Inventors: Donaldson, Lloyd E.
• Primary Examiner(s): Cashion, Jr., Merrell C.
• Assistant Examiner(s): WESSENDORF, TERESA D
• Application Number:
  US07/668,809
• Time in Patent Office: 613 Days
• Field of Search: 514/12, 514/14, 514/2, 514/800, 514/15, 530/313, 530/328, 930/130
• US Class Current: 514/9.9
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/59 Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]

Abstract

A hormone composition for producing superovulation in mammals. The composition has a particular ratio of follicle stimulating hormone (FSH) and luteinizing hormone (LH) which produces an optimum ovulation response in mammals and promotes out of season breeding and twinning. The composition can be produced from mammal pituitary glands or by recombinant DNA procedures and can be preserved in a phosphate buffered saline solution of thymol.

Claims

1. A composition of matter for producing superovulation in a domestic animal selected from the group consisting of sheep, goats, swine, horses and cattle comprising:

an effective amount of follicle stimulation hormone and lutenizing hormone, wherein the ratio of follicle stimulation hormone to lutenizing hormone is in a range of from about 500 to about 30,000 to 1, and a pharmaceutical solution of saline or phosphate buffered saline and an effective amount of an antimicrobial preservative comprising 5-methyl-2(1-methylethy)phenol.

2. The composition of matter of claim 1 wherein the ratio of follicle stimulation hormone to lutenizing hormone is in the range of from about 1,000 to about 23,000:

1.

3. The composition of claim 1, wherein said preservative 5-methyl-2(1-methylethyl)phenol comprises at least 0.04% by weight of the pharmaceutical solution.

4. The composition of claim 1, wherein said preservative 5-methyl-2(1-methylethyl)phenol comprises about 0.05 to about 0.1% by weight of the pharmaceutical solution.

5. A composition of matter for producing superovulation in goats comprising:

an effective amount of follicle stimulation hormone and luteinizing hormone, wherein the ratio of follicle stimulation hormone to luteinizing hormone is in a range of from about 500 to about 30,000 to 1.

6. A composition of matter for producing superovulation in swine comprising:

an effective amount of follicle stimulation hormone and luteinizing hormone, wherein the ratio of follicle stimulation hormone to luteinizing hormone is in a range of from about 500 to about 30,000 to 1.

7. A composition of matter for producing superovulation in sheep comprising:

an effective amount of follicle stimulation hormone and luteinizing hormone, wherein the ratio of follicle stimulation hormone to luteinizing hormone is in a range of from about 500 to about 30,000 to 1.

8. A composition of matter for producing superovulation in horses comprising:

an effective amount of follicle stimulation hormone and luteinizing hormone, wherein the ratio of follicle stimulation hormone to luteinizing hormone is in a range of from about 500 to about 30,000 to 1.

9. A method for producing superovulation in a domestic animal selected from the group consisting of sheep, goats, swine, horses and cattle comprising administering to said domestic animal a composition of an effective amount of follicle stimulating hormone and lutenizing hormone, wherein the ratio of follicle stimulating hormone to lutenizing hormone is in a range of from about 500 to about 30,000 to 1.

10. The method for producing superovulation in a domestic animal according to claim 9, wherein said composition is administered to the domestic animal by parenteral injection.

11. The method for producing superovulation in a domestic animal according to claim 10, wherein said composition is administered to the domestic animal in eight (8) equal doses at approximately twelve (12) hour intervals.

12. A method for achieving out of season breeding for a domestic animal selected from the group consisting of sheep, goats, swine, horses and cattle characterized as having breeding seasons comprising:

administering to the seasonal breeding domestic animal a composition of matter comprising an effective amount of follicle stimulating hormone and lutenizing hormone, wherein the ratio of follicle stimulating hormone to lutenizing hormone is in a ratio of from about 500 to about 30,000 to 1.

13. The method according to claim 12 wherein the domestic animal is selected from the group consisting of cattle, sheep, goats and horses.