Collagen-polymer conjugates

• US 5,162,430 A
• Filed: 11/14/1989
• Issued: 11/10/1992
• Est. Priority Date: 11/21/1988
• Status: Expired due to Term

• Current Assignee: Angiodevice International GmbH
• Original Assignee: Collagen Corp. (Abbvie Incorporated)
• Inventors: Wallace, Donald G., Bentz, Hanne, DeLustro, Frank, Michaels, Alan S., Rhee, Woonza, Fries, Louis, Burns, Ramon A. Jr.
• Primary Examiner(s): Nutter, Nathan M.
• Application Number:
  US07/433,441
• Time in Patent Office: 1,092 Days
• Field of Search: 525/54.1, 530/356, 530/840, 3/1, 128/DIG. 8, 604/891, 523/113
• US Class Current: 525/54.1
• CPC Class Codes: A61F 2/07 Stent-grafts
• CPC Class Codes: A61F 2310/00365 Proteins; Polypeptides; Degradation products thereof
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 47/60 the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• CPC Class Codes: A61K 47/61 the organic macromolecular compound being a polysaccharide or a derivative thereof
• CPC Class Codes: A61K 47/6435 the peptide or protein in the drug conjugate being a connective tissue peptide, e.g. collagen, fibronectin or gelatin
• CPC Class Codes: A61L 15/225 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2300/426 Immunomodulating agents, i.e. cytokines, interleukins, interferons
• CPC Class Codes: A61L 2300/62 Encapsulated active agents, e.g. emulsified droplets
• CPC Class Codes: A61L 2300/802 Additives, excipients, e.g. cyclodextrins, fatty acids, surfactants
• CPC Class Codes: A61L 24/102 Collagen
• CPC Class Codes: A61L 2430/16 for reconstruction of eye parts, e.g. intraocular lens, cornea
• CPC Class Codes: A61L 26/0033 Collagen
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/20 Polysaccharides
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/44 : having a macromolecular matrix
• CPC Class Codes: A61L 27/54 : Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 29/043 : Polysaccharides
• CPC Class Codes: A61L 29/045 : Collagen
• CPC Class Codes: A61L 29/06 : obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 29/16 : Biologically active materials, e.g. therapeutic substances A61L29/048 takes precedence
• CPC Class Codes: A61L 31/041 : Mixtures of macromolecular compounds
• CPC Class Codes: A61L 31/042 : Polysaccharides
• CPC Class Codes: A61L 31/044 : Collagen
• CPC Class Codes: A61L 31/10 : Macromolecular materials
• CPC Class Codes: A61L 31/125 : having a macromolecular matrix
• CPC Class Codes: A61P 17/00 : Drugs for dermatological disorders
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/495 : Transforming growth factor [TGF]
• CPC Class Codes: C07K 14/78 : Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C08B 37/0069 : Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
• CPC Class Codes: C08B 37/0072 : Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
• CPC Class Codes: C08H 1/06 : derived from horn, hoofs, hair, skin or leather
• CPC Class Codes: C08L 2666/02 : Organic macromolecular compounds, natural resins, waxes or and bituminous materials
• CPC Class Codes: C08L 5/00 : Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
• CPC Class Codes: C08L 71/02 : Polyalkylene oxides
• CPC Class Codes: C08L 89/00 : Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00
• CPC Class Codes: C08L 89/06 : derived from leather or skin , e.g. gelatin
• CPC Class Codes: C09J 189/06 : derived from leather or skin
• CPC Class Codes: G02B 1/043 : Contact lenses
• CPC Class Codes: Y10S 525/937 : Utility as body contact e.g. implant, contact lens or I.U.D.

Abstract

Collagen, particularly atelopeptide collagen, exhibits improved handling characteristics when chemically conjugated and/or crosslinked with a synthetic hydrophilic polymer.

Claims

1. A pharmaceutically acceptable, non-immunogenic composition comprising atelopeptide collagen chemically conjugated to a synthetic hydrophilic polymer.

2. The composition of claim 1 wherein the atelopeptide collagen is selected from the group consisting of type I, type II and type III.

3. The composition of claim 1 wherein said polymer is polyethylene glycol having a weight average molecular weight of about 400 to about 20,000.

4. The composition of claim 1 wherein said synthetic hydrophilic polymer is bound to an available lysine residue on said collagen.

5. The composition of claim 4 wherein said polymer has a first end and a second end, said first end being bound to said lysine residue, and said second end being non-bound.

6. The composition of claim 5 wherein synthetic hydrophilic polymer molecules are bound to 10-50% of said available lysine residues.

7. The composition of claim 6 wherein said synthetic hydrophilic polymer molecules are bound to 20-30% of said available lysine residues.

8. The composition of claim 4 wherein said polymer has a first end and a second end, wherein said first and second ends are bound to collagen.

9. The composition of claim 8 wherein the ratio of collagen molecules to polymer molecules is about 1:

1 to about 1;

20.

10. The composition of claim 8 wherein said collagen is atelopeptide fibrillar collagen or nonfibrillar collagen.

11. The composition of claim 1, wherein the synthetic hydrophilic polymer is polyethylene glycol.

12. The composition of claim 11 wherein the composition is a pharmaceutically acceptable injectable composition.

13. The composition of claim 1, wherein the synthetic hydrophilic polymer is polyethylene glycol having a molecular weight in the range of about 400 to about 20,000 and the composition is a pharmaceutically acceptable injectable composition.

14. A composition comprising:

collagen chemically conjugated to a synthetic hydrophilic polymer having a first end and a second end, said first end being bound to a collagen molecule, and said second end being bound to a growth factor.

15. The composition of claim 14 wherein said growth factor is selected from the group consisting of epidermal growth factor, transforming growth factor-α

, transforming growth factor-β

, transforming growth factor-β

1, transforming growth factor-β

2, platelet-derived growth factor-AA, platelet-derived growth factor-AB, platelet-derived growth factor-BB, acidic fibroblast growth factor, basic fibroblast growth factor, connective tissue activating peptide, β

-thromboglobulin, insulin-like growth factors, tumor necrosis factor, interleukins, colony stimulating factors, erythropoietin, nerve growth factor, interferons, and osteogenic factors.

16. The composition of claim 15 wherein said growth factor is selected from the group consisting of transforming growth factor-β

, transforming growth factor-β

1, transforming growth factor-β

2, and erythropoietin.

17. A pharmaceutically acceptable, non-immunogenic composition suitable for augmentation of soft tissue, which comprises:

atelopeptide collagen chemically conjugated to a synthetic hydrophilic polymer; and

a sufficient amount of a fluid pharmaceutically acceptable carrier.

18. The composition of claim 17 wherein the polymer is polyethylene glycol and the collagen is selected from the group consisting of non-fibrillar collagen and fibrillar collagen.

19. The composition of claim 17 wherein said polymer is monomethyl-polyethylene glycol having a weight average molecular weight of about 1,900 to about 8,000.

20. The composition of claim 19 wherein said monomethyl-polyethylene glycol is chemically conjugated to 10-50% of the available lysine residues on said collagen.

21. The composition of claim 17 which further comprises a tissue growth-promoting amount of epidermal growth factor, transforming growth factor-β

, an osteoinductive factor, or a hematopoietic factor.

22. The composition of claim 17 wherein said polymer has a first end and a second end, wherein said first and second ends are bound to collagen to form a crosslinked collagen-polymer conjugate, said composition having the form of a suspension of collagen-polymer conjugate particles in a pharmaceutically acceptable carrier.

23. The composition of claim 22 which further comprises a tissue growth-promoting amount of a growth factor selected from the group consisting of epidermal growth factor, transforming growth factor-β

, or a hematopoietic factor.

24. The composition of claim 23 wherein said factor is chemically bound to said collagen by a covalent bond between said factor and the first end of said polymer, and a covalent bond between the second end of said polymer and said collagen.

25. The composition of claim 17 wherein said fluid carrier is present in an amount sufficient to render the composition malleable.

26. The composition of claim 17 wherein said fluid carrier is present in an amount sufficient to render the composition injectable.

27. A pharmaceutically acceptable, non-immunogenic, injectable composition suitable for augmentation of soft tissue, which comprises:

atelopeptide collagen;

a synthetic hydrophilic polymer having a first and second end, wherein said first and second ends comprise reactive groups capable of forming a covalent bond in situ with an available lysine side chain present in collagen; and

a fluid pharmaceutically acceptable carrier in an amount sufficient to form an injectable composition.

28. The composition of claim 27 wherein said polymer comprises polyethylene glycol having a weight average molecular weight of about 400 to about 20,000.

29. The composition of claim 28 wherein said reactive groups comprise N-hydroxysuccinimide esters.

30. The composition of claim 27 wherein said composition comprises about 10 to about 100 mg/mL collagen and about 0.1 to about 30% synthetic hydrophilic polymer having reactive groups.

31. The composition of claim 30 wherein said composition comprises about 30 to about 80 mg/mL collagen and about 0.3 to about 10% synthetic hydrophilic polymer having reactive groups.

32. The composition of claim 27 wherein said collagen comprises fibrillar atelopeptide collagen.

33. The composition of claim 27 wherein said collagen comprises nonfibrillar collagen.