Detection apparatus for electrophoretic gels
First Claim
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1. An instrument for the detection of the fluorescence pattern emitted by biological molecules on an electrophoresis gel which are tagged with one of four fluorophores comprising:
- excitation means to excite the fluorophores on the molecules;
an interference filter assembly which includes four interference filters selected to pass light in a wavelength band corresponding to the emission characteristics of the fluorophores;
a prism wedge assembly which includes a respective prism wedge optically associated with each of the interference filters in the interference filter assembly to bend the light signal from the associated interference filter so that the light signals emitted by the four interference filters are separated from each other;
a light detector for detecting light incident thereon and for determining the relative intensity of the light incident thereon; and
optical focusing means to direct fluorescence from the gel through the interference filter assembly and then through the prism wedge assembly to the detector so that the light impinging on the detector is separated into four bands, each of which is related to the intensity of the fluorescence from the gel in one of the wavelength bands associated with one of the interference filters.
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Abstract
An apparatus is disclosed to optically determine which of four fluorophores is attached to a band of DNA molecules on an electrophoresis gel. The apparatus includes four separate band pass interference filters and four wedge prisms to create four discrete areas of light on a detector. Digital comparison of the relative intensity of light sensed in the four discrete areas determines the identity of the excited fluorophore.
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Citations
6 Claims
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1. An instrument for the detection of the fluorescence pattern emitted by biological molecules on an electrophoresis gel which are tagged with one of four fluorophores comprising:
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excitation means to excite the fluorophores on the molecules; an interference filter assembly which includes four interference filters selected to pass light in a wavelength band corresponding to the emission characteristics of the fluorophores; a prism wedge assembly which includes a respective prism wedge optically associated with each of the interference filters in the interference filter assembly to bend the light signal from the associated interference filter so that the light signals emitted by the four interference filters are separated from each other; a light detector for detecting light incident thereon and for determining the relative intensity of the light incident thereon; and optical focusing means to direct fluorescence from the gel through the interference filter assembly and then through the prism wedge assembly to the detector so that the light impinging on the detector is separated into four bands, each of which is related to the intensity of the fluorescence from the gel in one of the wavelength bands associated with one of the interference filters. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification