DNA sequences encoding the osteoinductive proteins

• US 5,166,058 A
• Filed: 07/11/1989
• Issued: 11/24/1992
• Est. Priority Date: 07/01/1986
• Status: Expired due to Term

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Wozney, John M., Wang, Elizabeth A., Rosen, Vicki A.
• Primary Examiner(s): Ellis, Joan
• Application Number:
  US07/378,537
• Time in Patent Office: 1,232 Days
• Field of Search: 435/69.1, 435/41, 435/172.3, 435/235, 435/240.1, 435/252.3, 435/320.1, 435/235.1, 435/240.2, 435/255, 435/256, 435/252.33, 556/27, 538/350, 935/18, 935/27, 935/31, 935/34, 935/38, 935/55, 935/62, 935/72, 935/70, 935/81
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 24/043 Mixtures of macromolecular materials
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

Purified BMP-2 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.

Claims

1. An isolated and purified DNA sequence encoding an osteoinductive protein said DNA sequence comprising the nucleotide sequence form nucleotide #356 to #1543 of FIG. 2.

3. A vector comprising the DNA sequence of claim 1.

5. A host cell transformed with the vector of claim 3.

7. A method for producing a purified BMP-2A protein said method comprising the steps of

(a) culturing in a suitable culture medium said transformed host cells of claim 5;

and

(b) isolating and purifying said BMP-2A from said culture medium.

2. An isolated and purified DNA sequence encoding an osteoinductive protein said DNA sequence comprising the nucleotide sequence from nucleotide #403 to #1626 of FIG. 3.

4. A vector comprising a DNA sequence of claim 2.

6. A host cell transformed with he vector of claim 4.

8. A method for producing a purified BMP-2B protein said method comprising the steps of

(a) culturing in a suitable culture medium said transformed host cells of claim 6;

and

(b) isolating and purifying said BMP-2B from said culture medium.

9. A method for producing a purified BMP-2A protein said method comprising the steps of

(a) culturing in a suitable culture medium a mammalian host cell transformed with a DNA sequence comprising nucleotide #356 through #1543 of FIG. 2;

and

(b) isolating and purifying said BMP-2A from said culture medium.

10. The method of claim 9 wherein said mammalian host cell is a CHO cell.

11. A method for producing a purified BMP-2B protein said method comprising the steps of

(a) culturing in a suitable culture medium a mammalian host cell transformed with a DNA sequence comprising nucleotide #403 through #1626 of FIG. 3;

and

(b) isolating and purifying said BMP-2B from said culture medium.

12. The method of claim 11 wherein said mammalian host cell is a CHO cell.