Measurement of blood coagulation time using infrared electromagnetic energy

• US 5,167,145 A
• Filed: 09/19/1990
• Issued: 12/01/1992
• Est. Priority Date: 09/19/1990
• Status: Expired due to Term

• Current Assignee: David M. Butler, Harold B. Kirkpatrick, John H. Staehlin
• Original Assignee: David M. Butler, Harold B. Kirkpatrick, John H. Staehlin
• Inventors: Butler, David M., Staehlin, John H., Kirkpatrick, Harold B.
• Primary Examiner(s): Williams, Hezron E.
• Assistant Examiner(s): Brock, Michael J.
• Application Number:
  US07/584,789
• Time in Patent Office: 804 Days
• Field of Search: 73/64.1, 250/564, 250/573, 250/576, 356/39, 436/69, 422/73
• US Class Current: 73/64.43
• CPC Class Codes: G01N 33/4905 Determining clotting time of blood by chemical methods G01N33/86, C12Q1/54

Abstract

A method and apparatus for detection of blood coagulation using infrared electromagnetic transmission changes through a sample from a source of infrared energy to suitable detection electronics producing a peak signal representative of the clotting time.

Claims

1. A method recording the clotting time of whole blood as it proceeds from fibrinogen through known stages of coagulation, culminating in the cross-linking of the fibrin clot which ultimately solidifies and dries at the end of a cycle, including the steps of:

providing a measured sample of whole blood to be tested;

transmitting infrared electromagnetic energy through the blood sample;

detecting the transmission of the infrared energy and electronically producing the signals in response to said infrared energy; and

measuring a peak transmission level of said infrared energy and calibrating said peak signal in terms of clotting time of said whole blood sample whereby the clotting time is represented by the peak transmission level of the infrared energy through the whole blood sample.

2. The method of claim 1 wherein the step of providing a sample includes drawing blood from the fingertip of a subject and depositing the sample in a receptacle using a delivery capillary tube fitted with a rubber bulb which delivers the sample to the receptacle when it is squeezed by the instrument operator.

3. The method of claim 2 further including the step of inhibiting the clotting process by use of a plastic capillary delivery tube until the sample is deposited within said receptacle.

4. The method of claim 1 wherein the sample deposited in the receptacle is whole blood.

5. An instrument for measuring and recording the clotting time of blood by measuring changes in the amplitude of an electromagetic energy transmission through the blood coinciding with changes in the composition of the blood as it proceeds from fibrinogen through known stages of coagulation culminating in the cross-linking of the fibrin clot which ultimately solidifies and dries at the end of a cycle represented by a peak amplitude of the electromagnetic energy transmission comprising:

a measured sample to be tested;

means for transmitting a source of infrared electromagnetic energy through said sample;

detector means for measuring changes in amplitude of said infrared electromagnetic energy signal;

circuit means for providing a signal representative of said changes in amplitude; and

,

means for producing a signal calibrated in terms of coagulation time when peak infrared transmission is recorded.

6. The instrument of claim 5 wherein said transmitting means is an infrared light emitting diode.

7. The instrument of claim 5 further including means to record said signal to provide a permanent record thereof.

8. The instrument of claim 7 further including power supply means for providing power to said instrument.

9. The instrument of claim 5 further including analog circuitry for detecting said changes in amplitude, analog to digital convertor for providing a digital output representative of said amplitude changes, digital processor means for providing an output signal display means and recording means connected to said digital processor to display and record said signals in terms of coagulation time.

10. The instrument of claim 5 further including control panel means and switch means actuated in response to the insertion of a blood sample in a sample holding means;

whereby actuation of said switch means begins a test cycle.

11. An instrument for measuring and recording clotting time of blood by measuring changes in amplitude of an infrared energy transmission through a blood sample coinciding with changes in the composition of the blood as it proceeds from fibrinogen through known stages of coagulation culminating in the cross-linking of a fibrin clot which ultimately solidifies and dries at the end of the cycle represented by a peak amplitude of the infrared energy transmission, comprising:

means for holding a measured sample to be tested;

means for transmitting a source of infrared electromagnetic energy through the sample;

detector means for measuring changes in amplitude of said infrared electromagnetic energy signal;

circuit means for providing a signal representative of said changes in amplitude;

means for producing a signal calibrated in terms of coagulation time when peak infrared transmission is recorded; and

a cylindrical channel extending from the exterior of the instrument to a point adjacent said sample holding means, said channel constructed to receive a cylindrical capillary delivery tube to precisely deposit and locate said sample to be tested on said sample holding means.

12. The instrument of claim 11 further including switching means adjacent said cylindrical channel and operable in response to said capillary delivery tube, said switch means being connected to a suitable control means for beginning the operation of a test cycle.

13. The instrument of claim 11 in which the blood sample holding means and said cylindrical capillary delivery tube are both disposable.