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Method for determination of glucose concentration

  • US 5,168,046 A
  • Filed: 07/05/1990
  • Issued: 12/01/1992
  • Est. Priority Date: 07/13/1989
  • Status: Expired due to Term
First Claim
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1. A method for the in vitro measurement of the concentration of glucose in a whole blood sample, which comprises:

  • A) obtaining a calibration curve relating (a) a conversion factor, defined as the ratio of the measured glucose concentration in a whole blood sample to the measured glucose concentration of the serum from said whole blood sample to (b) the proportion of the whole blood volume that consists of the serum fraction;

    B) creating a calibration curve relating (a) the ratio of glucose concentration in said whole blood sample measured before glucose has diffused out of the cells into a solution used to dilute said whole blood sample to the glucose concentration measured in said whole blood sample after glucose has diffused out of the cells into said solution to (b) the proportion of the whole blood volume that consists of the serum fraction;

    C) diluting a whole blood sample in which the glucose concentration is to be determined in a solution suitable for measuring glucose concentration;

    D) obtaining a first value (Cy) of the concentration of glucose in the sample solution of step (C) before the glucose contained within the cells has diffused out of the cells into the solution used to dilute the sample;

    E) obtaining a second value (Cx) of the concentration of glucose in the sample solution of step (C) after the glucose contained within the cells has diffused out of the cells into the solution used to dilute the sample;

    F) obtaining the ratio of Cx/Cy from the values obtained in steps (D) and (E);

    G) estimating the proportion of the whole blood volume of said whole blood sample of step (C) which consists of the serum fraction (X) by reference to the calibration curve obtained in step (B);

    H) obtaining the value for the conversion factor at the value of X determined in step (G) by reference to the calibration curve obtained in step (A); and

    I) determining the concentration of glucose in the whole blood sample of step (C) by multiplying the value of Cx obtained in step (E) by the value of the conversion factor obtained in step (H).

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