Interferometric measurement of glucose by refractive index determination

• US 5,168,325 A
• Filed: 02/28/1990
• Issued: 12/01/1992
• Est. Priority Date: 02/28/1990
• Status: Expired due to Fees

• Current Assignee: Michigan Technological University
• Original Assignee: Michigan Technological University
• Inventors: Yoder-Short, Dale R.
• Primary Examiner(s): Turner, Samuel A.
• Application Number:
  US07/486,242
• Time in Patent Office: 1,007 Days
• Field of Search: 356/36, 356/39, 356/361, 356/12 B
• US Class Current: 356/517
• CPC Class Codes: G01N 21/45 using interferometric methods; using Schlieren methods

Abstract

An apparatus and method for testing the glucose level in a blood sample is provided. The method includes the step of initially filtering a sample of blood to be tested. A beam of light is provided and split into a pair of beams by a beam splitter. The pair of beams travel along generally parallel paths. One path contains a cell of a known optical pathlength and additionally a compensator. The other path has a cell containing the filtered blood sample to be tested. The pair of beams are then recombined by a mirror and a fringe pattern is detected at a detector. From the fringe pattern, the refractive index of the blood sample can be calculated. The refractive index is easily converted to a specific glucose concentration. Alternatively, the original fringe pattern can be restored by changing the effect of the compensator. The refractive index can then be found from the required compensation.

Claims

1. A method of measuring the glucose level in a blood sample comprising the steps of:

placing a known solution into a first cell and second cell;

providing a light source for generating an original beam of light;

splitting the original beam of light into a first and second beam;

passing the first beam through the first cell while simultaneously passing the second beam through the second cell;

recombining the first and second beams into a final beam to establish a fringe pattern;

filtering a blood sample to remove all components exceeding a predetermined weight and placing the filtered blood sample into the first cell;

measuring a shift in the fringe pattern to determine the refractive index of the filtered blood sample.

2. A method as set forth in claim 1 including the step of utilizing a white light source for providing the original beam.

3. A method of measuring the glucose level in a blood sample comprising the steps of:

placing a known solution into a first cell and a second cell;

providing a light source for generating an original beam of light;

splitting the original beam of light into a first and second beam;

passing the first beam through the first cell while simultaneously passing the second beam through the second cell;

recombining the first and second beam into a final beam to establish a fringe pattern;

filtering a blood sample to remove all components exceeding a predetermined weight;

placing the filtered blood sample into the first cell;

measuring a refractive index of the filtered blood sample based on the fringe pattern; and

calculating the glucose level in the filtered blood sample based on the measured refractive index.

4. A method of measuring the glucose level in a blood sample comprising the steps of:

placing a known solution into a first and second cell;

providing a light source for generating an original beam of light;

splitting the original beam of light into a first and second beam;

passing the first beam through the first cell while simultaneously passing the second beam through the second cell;

passing the second beam of light through a compensator;

recombining the first and second beams into a final beam to establish a fringe pattern;

filtering a blood sample to remove all components exceeding a predetermined weight;

placing the filtered blood sample into the first cell; and

measuring the refractive index of the filtered blood sample based on the fringe pattern.

5. A method of measuring the glucose level in a blood sample comprising the steps of:

placing a known solution into a first and second cell;

providing a light source for generating an original beam of light;

splitting the original beam of light into a first and second beam;

passing the first beam through the first cell while simultaneously passing the second beam through the second cell and a compensator;

recombining the first and second beams into a final beam to establish an original fringe pattern;

filtering a blood sample to remove all components exceeding a predetermined weight;

placing the filtered blood sample in the first cell;

calculating the glucose level in the filtered blood sample by adjusting the compensator to restore the original fringe pattern.

6. A method of measuring the glucose level in a blood sample comprising the steps of:

placing a known solution into a first and second cell;

providing a light source for generating an original beam of light;

splitting the original beam of light into a first and second beam;

passing the first beam through the first cell while simultaneously passing the second beam through the second cell;

recombining the first and second beams into a final beam to establish a fringe pattern;

filtering the blood sample to remove all components having a molecular weight exceeding one thousand daltons;

placing the filtered blood sample into the first cell; and

measuring the refractive index of the filtered blood sample based on the fringe pattern.

7. A method of measuring the glucose level of blood comprising the steps of:

filtering a sample of blood to remove cells and proteins exceeding a predetermined weight;

providing a light source for generating an original beam;

splitting the original beam into first and second beams;

placing a first cell containing the filtered blood sample into the first beam of light;

placing a second cell in the second beam;

recombining the beams of light after each has passed through its respective cell to form a final beam; and

measuring the fringe pattern of the final beam to determine the refractive index of the blood sample.

8. A method of measuring the glucose level of blood comprising the steps of:

filtering a sample of blood to remove cells and proteins exceeding a predetermined weight;

providing a light source for generating an original beam;

splitting the original beam into first and second beams;

placing a first cell containing the filtered blood sample into the first beam of light;

placing a second cell in the second beam;

recombining the beams of light after each has passed through its respective cell to form a final beam; and

restoring the original fringe pattern using a compensator to determine the refractive index of the blood sample.

9. A device for measuring the glucose level in a blood sample comprising:

a light source for producing a beam of light;

a first mirror for separating the beam of light into a first beam and a second beam;

a first cell containing a blood sample positioned in said first beam;

a second cell containing a known solution positioned in said second beam;

a second mirror for recombining said first beam and said second beam into a single light beam; and

a photodetector for detecting the intensity of the recombined beam to determine the refractive index and glucose level of the blood sample.

10. The device of claim 9 including compensator means adjustable to compensate for the difference in path lengths of said first beam and said second beam to make the path length of said second beam the same as the path length of said first beam.

11. The device of claim 9 wherein said light source is a white light source.