Method of detecting angle of optical rotation in solution having time-dependent concentration, detection apparatus therefor, and detector cell therefor
First Claim
1. A method of detecting an angle of optical rotation caused by an optical rotary power of substance contained in a flowing sample solution, comprising the steps of:
- i) generating a first beam consisting of linearly polarized light and a second beam having an impartial polarization direction,ii) transmitting the first beam and second beam through the sample solution flowing through a measurement passage of a flow cell,iii) measuring the light intensity of at least one polarized light component of the transmitted light in at least one direction,iv) obtaining a first signal corresponding to an angle of rotation of the sample solution against the first beam from the light intensity of the polarized light component of the transmitted light of the first beam,v) obtaining a second signal corresponding to an angle of rotation of the sample solution against the second beam from the light intensity of the polarized light component of the transmitted light of the second beam, andvi) subtracting the second signal from the first signal to obtain a signal corresponding to the angle of optical rotation caused by an optical rotary power of substance contained in the sample solution.
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Accused Products
Abstract
In a detection apparatus, a linearly polarized light beam polarized in a polarizer (62) is converted by being transmitted through a Pockels cell (60), driven by an alternative voltage, into an alternative linearly polarized light beam and a circularly polarized light beam, which is transmitted through a sample solution flowing through a flow cell (68). The transmitted light beam is separated into two polarized light components, and an intensity thereof is measured to obtain a signal proportional to an angle of rotation by the sample solution. A signal corresponding to an angle of rotation caused by an optical rotary power of the sample solution is obtained by subtracting the signal obtained during irradiation of the circularly polarized light from the signal obtained during irradiation of the linearly polarized light. The sample solution (18) is infused into an optical path of the flow cell (68) under a condition where mean velocity is 0.5 to 5 m/sec.
40 Citations
16 Claims
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1. A method of detecting an angle of optical rotation caused by an optical rotary power of substance contained in a flowing sample solution, comprising the steps of:
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i) generating a first beam consisting of linearly polarized light and a second beam having an impartial polarization direction, ii) transmitting the first beam and second beam through the sample solution flowing through a measurement passage of a flow cell, iii) measuring the light intensity of at least one polarized light component of the transmitted light in at least one direction, iv) obtaining a first signal corresponding to an angle of rotation of the sample solution against the first beam from the light intensity of the polarized light component of the transmitted light of the first beam, v) obtaining a second signal corresponding to an angle of rotation of the sample solution against the second beam from the light intensity of the polarized light component of the transmitted light of the second beam, and vi) subtracting the second signal from the first signal to obtain a signal corresponding to the angle of optical rotation caused by an optical rotary power of substance contained in the sample solution. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of detecting an angle of optical rotation caused by an optical rotary power of substance contained in a flowing sample solution comprising the steps of:
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ii) generating at least one beam including a linearly polarized light beam, iii) transmitting the beam through the sample solution flowing through a measurement passage of a flow cell, iv) measuring the light intensity of at least one polarized light component of the transmitted light in at least one direction, and v) obtaining a signal corresponding to the angle of rotation of the sample solution from the light intensity of the polarized light component, and further comprising the step of vi) infusing the sample solution into the measurement passage at a mean velocity of 0.5 to 5 m/sec.
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8. An apparatus for detecting an angle of optical rotation caused by an optical rotary power of substance contained in a flowing sample solution comprising:
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means for generating a first beam of linearly polarized light and a second beam having an impartial polarization direction; a flow cell having a measurement passage through which the sample solution transmitting the first beam and the second beam can flow; means for measuring a light intensity of at least one polarized light component of the transmitted light in at least one direction; and calculating means for calculating a value corresponding to the angle of rotation of the sample solution from the light intensity of the transmitted light, and for subtracting the value obtained during irradiation of the second beam from the value obtained during irradiation of the first beam to obtain the angle of optical rotation caused by an optical rotary power of substance contained in the sample solution. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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15. An apparatus for detecting an angle of optical rotation caused by an optical rotary power of substance contained in a flowing sample solution comprising:
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means for generating at least one beam including a linearly polarized light beam; a flow cell having a measurement passage through which the sample solution transmitting the beam can flow; means for measuring the light intensity of at least one polarized light component of the transmitted light in at least one direction; and calculating means for calculating the angle of rotation of the sample solution from the light intensity of the polarized light component, wherein the flow cell also has an infusion passage for infusing the sample solution into the measurement passage at a mean velocity of 0.5 to 5 m/sec. - View Dependent Claims (16)
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Specification