Ion-permeable polymer joint for use in capillary electrophoresis
First Claim
1. A method of producing a capillary electrophoresis grounding joint, comprising the steps of:
- providing an elongated electrophoretic capillary tube;
scoring said tube at a region thereof intermediate the ends thereof;
placing a sleeve of ion-permeable material over said score region; and
fracturing said tube at said score region.
4 Assignments
0 Petitions
Accused Products
Abstract
Improved capillary electrophoresis (CE) apparatus (10) is provided which includes an intermediate grounding joint (34) serving to isolate delicate electrochemical detectors (32, 54, 58) from the high voltage CE power supply (20). The joint (34) includes a fracture (36) close to the detection end (22) of the apparatus (10) which is covered by a Nafion sleeve (38). The joint (34) is immersed in an ionic buffer reservoir (46) which includes a grounded cathode (48). In fabrication procedures, a capillary tube (12) is scored in proximity to one end (22) thereof, and the Nafion sleeve (38) is slid over the score line. The sleeve (38) is then sealed with epoxy (40, 42) at the ends thereof.
15 Citations
18 Claims
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1. A method of producing a capillary electrophoresis grounding joint, comprising the steps of:
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providing an elongated electrophoretic capillary tube; scoring said tube at a region thereof intermediate the ends thereof; placing a sleeve of ion-permeable material over said score region; and fracturing said tube at said score region. - View Dependent Claims (2, 3, 4, 5)
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6. Capillary electrophoresis apparatus comprising:
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an elongated electrophoretic capillary tube assembly presenting a pair of opposed ends respectively adapted for operative connection with detecting means and a power supply for applying a high voltage potential across the tube assembly; and means for electrically isolating said detecting means from said applied potential, including structure defining an opening in said tube assembly intermediate said ends thereof, and a cover applied to said tube assembly in covering relationship to said opening, said opening and cover being cooperable for creating an electrically conductive path to ground when the isolating means is immersed in a grounded ionic bath, said cover being formed of flexible polymeric material which is ion-permeable and of low electrical resistance. - View Dependent Claims (7, 8, 9, 10, 11)
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12. Capillary electrophoresis apparatus, comprising:
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elongated electrophoretic capillary tube assembly presenting a pair of opposed ends; a power supply operatively coupled with one of said ends for applying a high voltage across the tube assembly; electrophoretic detection means operably coupled with the opposed end of said tube assembly; and means for electrically isolating said detecting means from said applied potential, including structure defining an opening in said tube assembly intermediate said ends thereof, and a cover applied to said tube assembly in covering relationship to said opening, said opening and cover being cooperable for creating an electrically conductive path to ground when the isolating means is immersed in a grounded ionic bath, said cover being formed of flexible polymeric material which is ion-permeable and of low electrical resistance. - View Dependent Claims (13, 14, 15, 16, 17, 18)
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Specification