Treated corneal prosthetic device

• US 5,171,318 A
• Filed: 01/21/1992
• Issued: 12/15/1992
• Est. Priority Date: 11/09/1987
• Status: Expired due to Fees

• Current Assignee: Chiron Ophthalmics Incorporated
• Original Assignee: Chiron Ophthalmics Incorporated
• Inventors: Gibson, David, Lerner, Michael, Reich, Cary, Nordquist, Robert
• Primary Examiner(s): Isabella, David
• Assistant Examiner(s): BRITTINGHAM, DEBRA
• Application Number:
  US07/825,425
• Time in Patent Office: 329 Days
• Field of Search: 623/6, 623/66, 427/2
• US Class Current: 623/5.16
• CPC Class Codes: A61K 6/30 Compositions for temporarily or permanently fixing teeth or palates, e.g. primers for dental adhesives
• CPC Class Codes: A61K 6/887 Compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/404 Biocides, antimicrobial agents, antiseptic agents
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2300/606 Coatings
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: C08L 3/20 Oxidised amylose; Oxidised amylopectin
• CPC Class Codes: C08L 33/08 Homopolymers or copolymers of acrylic acid esters
• CPC Class Codes: C08L 33/10 Homopolymers or copolymers of methacrylic acid esters
• CPC Class Codes: C08L 39/06 Homopolymers or copolymers of N-vinyl-pyrrolidones
• CPC Class Codes: C08L 77/04 Polyamides derived from alpha-amino carboxylic acids C08L77/10 takes precedence
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

Prosthetic devices are prepared for subepithelial implantation by coating their surfaces with a coating comprising crosslinked or uncrosslinked fibronectin. If the surface of the device comprises a material which does not normally bind fibronectin, it is pretreated, for example, by coating with poly(L-lysine). The coated prosthetic device is implanted by removing a portion of the epithelium, affixing the coated device to the subepithelial tissue optionally replacing all or part of the removed epithelium, and allowing new epithelial tissue to grow over and affix itself to the surface of the prosthetic device. The method of the invention can be used to implant contact lenses permanently beneath the epithelium of the cornea.

Claims

1. A treated corneal prosthetic device for subepithelial implantation in a human or animal cornea comprising a prosthetic device having on its surface a coating comprising crosslinked or uncrosslinked fibronectin or a biologically active fragment or analog of fibronectin, the surface of the prosthetic device comprising a synthetic polymeric material which is pre-treated to chemically modify said material to make it capable of binding said fibronectin, fragment or analog, whereby the coating is supportive for corneal epithelial cells and supports the attachment, growth and migration of corneal epithelial tissues across the surface of the prosthetic device.

2. A device as claimed in claim 1 wherein the surface of the synthetic polymeric material is pretreated by coating with poly(L-lysine) to render it capable of binding fibronectin.

3. A device as claimed in claim 1 wherein the coating is a crosslinked coating comprising fibronectin and a crosslinking agent capable of crosslinking fibronectin.

4. A device as claimed in claim 3, wherein the crosslinking agent is a compound of the formula ##STR4## wherein R is selected from --CH₂ --_n, --S--S--, ##STR5## and --CH₂ --_n S--S--CH₂ --_n which n, is an integer having a value of at least 1;

R'"'"' and R", which can be the same or different are each individually alkoxy having from 1 to 20 carbon atoms; and

X^- is an anionic counterion.

5. A device as claimed in claim 4 wherein the crosslinking agent is dimethyl pimelimidate dihydrochloride.

6. A device as claimed in claim 1 wherein the surface of the synthetic polymeric material is pretreated with cyanogen bromide to render it capable of binding fibronectin.

7. A treated corneal prosthetic device for subepithelial implantation in a human or animal cornea comprising a prosthetic device having on its surface a coating comprising crosslinked or uncrosslinked fibronectin, the surface of the prosthetic device comprising a synthetic polymeric material which is pre-treated to chemically modify said material to make it capable of binding said fibronectin, whereby the coating is supportive for corneal epithelial cells and supports the attachment, growth and migration of corneal epithelial tissues across the surface of the prosthetic device.