Biological assay cassette and method for making same

• US 5,171,533 A
• Filed: 07/31/1989
• Issued: 12/15/1992
• Est. Priority Date: 07/31/1989
• Status: Expired due to Fees

• Current Assignee: Roche Diagnostics Corporation (Roche Holding AG)
• Original Assignee: Gerald L. Klein, John T. Kingsley, Richard A. Fine
• Inventors: Klein, Gerald L., Kingsley, John T., Fine, Richard A.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Blythe, Stephanie
• Application Number:
  US07/387,917
• Time in Patent Office: 1,233 Days
• Field of Search: 422/72, 422/101, 436/45, 436/177, 435/287, 210/782, 210/789, 210/516, 210/518
• US Class Current: 422/72
• CPC Class Codes: G01N 2035/00495 Centrifuges
• CPC Class Codes: G01N 2035/00564 Handling or washing solid phase elements, e.g. beads
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A modular reaction cassette has a fluid metering mechanism and valves for releasing fluid which are actuated by various centripetal acellerations generated in an automated patient analysis instrument having a variable speed rotor. Various geometries and sealant materials are used to select the rotational speed at which the sealant material yields to operate the metering device and release valves.

Claims

1. A centrifugal force responsive fluid metering device for use with biological assay cartridges, comprising:

a slidable metering member having a pre-determined mass and defining a metering cavity for metering a precise quantity of analyte in a fluid, the metering member being moveable from a first collecting position through a second sealed position to a third dispensing position;

a fluid metering chamber for slidably supporting the metering member and for containing the fluid to be metered, the metering chamber having a sealing surface slidably abutting the metering member so as to define a predetermined gap distance therebetween and having a surface area sufficiently large to completely enclose the metering cavity when the metering member is in the second sealed position and sufficiently small to permit the metering cavity to be filled with the fluid when the metering member is in the first collecting position and to permit the metering cavity to dispense the fluid therefrom when the metering member is in the third dispensing position; and

a sealing material positioned in the gap between the metering member and the sealing surface to substantially prevent fluid communication between the first fluid collecting and third fluid dispensing positions except for the metered quantity of fluid, the sealing material having a preselected shear strength, whereby the metering member slides from the first to third positions only upon application of a first predetermined centrifugal force thereto in a predetermined direction defined by the first, second and third positions. supplemental sealing means for sealing the fluid metering chamber when the metering member is in the third fluid dispensing.

2. The metering device of claim 1, including entrapment means for preventing the metering member from escaping from the metering chamber past the third dispensing position.

3. The metering device of claim 1, wherein the metering member has a substantially elongated cylindrical body and wherein the metering cavity is located in the side of the body, wherein the sealing surface in the fluid metering chamber is a substantially cylindrical surface of larger, substantially constant diameter than the metering member body so that the gap is annular and positioned so that the metering body and sealing surface define an axis aligned with the predetermined direction.

4. The metering device of claim 3 wherein the metering member includes an enlargement on one end thereof of seal the bore when the metering member enters the third dispensing position, thereby sealing the fluid metering chamber.

5. The metering device of claim 3, wherein the metering chamber is a substantially hemispherical divot.

6. The metering device of claim 3, wherein the sealing material is sufficiently compliant so as to reseal itself after shearing, and wherein the seal material has a lower sheer strength than adhesive strength.

7. The metering device of claim 6, wherein the gap distance is approximately 0.0025", wherein the area of the sealing surface abutting the metering member is approximately 1.183 cm², wherein the shear strength of the sealing material in the gap is approximately 382 dynes/cm², and wherein the metering member and the fluid to be contained in the metering cavity have a combined mass of approximately 0.34 gm, whereby the metering member will slide from the first fluid collecting to third fluid dispensing position upon application of a force of approximately 1454 gravities in the predetermined direction.

8. The metering device of claim 1, adapted so as to be useful for metering a precise volume of plasma from a whole blood sample introduced into the metering chamber including, a peripheral blood cell sump located in the metering chamber below the metering cavity in the direction of the predetermined centrifugal force, wherein the metering member is a cylindrical metering body and wherein the metering cavity is a transverse bore passing through the cylindrical metering body at an oblique angle with respect to the axis of the cylindrical metering body, whereby whole blood cells in the metering chamber and in the metering cavity will collect in the blood cell sump upon application of a centrifugal force in the axial direction leaving only blood plasma in the metering chamber to be disposed upon application of the predetermined centrifugal force.

9. The metering device of claim 1, including a first reaction chamber for containing a first reaction fluid, connected to and positioned axially with respect to the metering chamber so as to receive metered analyte dispensed therefrom, the reaction chamber having a centrifugal force-sensitive release valve therein including release means for opening the release valve at a second predetermined centrifugal force greater than the first predetermined centrifugal force.

10. The metering device of claim 9, wherein the centrifugal force-sensitive release valve has a cylindrical valve body having a preselected mass and is reciprocally moveable between open and closed positions in an axially directed bore defined by the reaction chamber, including a sealing material having a second preselected shear strength in an annular gap formed between the cylindrical valve body and the bore, whereby the centrifugal force-sensitive release valve will open and release the contents of the reaction chamber upon application of the second predetermined centrifugal force.

11. The metering device of claim 10, wherein the centrifugal force-sensitive release valve includes a tensile release structure having a radially directed sealing surface extending from the bore, a radially extending foot connected to the cylindrical valve body, and a sealing material having a preselected tensile strength between the radially directed sealing surface and the foot.

12. The metering device of claim 11, wherein the radially extending foot has a peripheral, frusto-conic skirt extending therefrom to distribute the contents of the first reaction chamber in a radially outward direction.

13. The metering device of claim 9, including a second reaction chamber for containing a second reaction fluid, connected to and connected axially with respect to the first reaction chamber so as to receive reacted analyte dispensed from the first reaction chamber, the second reaction chamber having a centrifugal force-sensitive release valve therein including release means for opening the release valve at a third predetermined centrifugal force greater than the first and second predetermined centrifugal force.

14. The metering device of claim 1, including a photometric analysis chamber connected to the metering chamber so as to receive metered analyte therefrom for photometric analysis in a fluid contained in the photometric analysis chamber, the photometric analysis chamber further including accumulation means for concentrating the population density of particles above a fluid interface in the photometric analysis chamber to facilitate the movement of the particles across a fluid meniscus formed at the fluid interface by centrifugal force.

15. The metering device of claim 1 including a second metering device connected in series therewith.

16. The metering device of claim 1, including supplemental sealing means for sealing the fluid metering chamber when the metering member is in the third fluid dispensing position.

17. A force responsive fluid metering cassette for performing biological assays and chemical reactions, comprising:

a metering chamber having means for accepting a fluid to be metered and means for precisely metering a portion of the fluid upon application of a first centrifugal force;

a first reaction chamber for containing a first reaction fluid, the reaction chamber being connected to the metering chamber and having means for receiving the metered fluid therefrom and means for releasing the contents of the reaction chamber upon application of a second predetermined centrifugal force greater than the first centrifugal force; and

a photometric analysis chamber connected to the first reaction chamber so as to receive the contents of the first reaction chamber therefrom for photometric analysis in a fluid contained in the photometric analysis chamber, the photometric analysis chamber further including accumulation means for concentrating the population density of particles above a fluid interface in the photometric analysis chamber to facilitate the movement of the particles across a fluid meniscus formed at the fluid interface by centrifugal force.

18. The metering device of claim 17, including a second reaction chamber for containing a second reaction fluid, connected between the first reaction chamber and the photometric analysis chamber so as to receive fluid dispensed from the first reaction chamber, the second reaction chamber having means for releasing the contents of the second reaction into the photometric analysis chamber upon application of a third predetermined centrifugal force, greater than the first and second centrifugal forces.

19. An acceleration responsive fluid metering cassette for performing biological assays and chemical reactions, comprising:

an enclosed reaction chamber having means for making a photometric determination of the contents therein;

a metering chamber within the reaction chamber, the metering chamber having means for accepting a fluid to be metered and means for precisely metering a portion of the fluid into the reaction chamber upon application of a first centrifugal force;

a first reaction fluid container in the reaction chamber for containing a first reaction fluid, the reaction fluid container having means for releasing the first reaction fluid upon application of a second predetermined centrifugal force greater than the first centrifugal force.

20. The metering device of claim 19, including a second fluid reaction container in the reaction chamber for containing a second reaction fluid, the second reaction fluid into the reaction chamber upon application of a third predetermined acceleration, greater than the first and second accelerations, by centrifugal force.

21. A centrifugal force-sensitive release valve, comprising:

a movable member having a predetermined mass and a sealing surface thereon;

a fixed member having an aperture therein sized to slidably receive the fixed member so as to form a peripheral gap therebetween in the approximate range of 0.002" to 0.020"; and

a sealing material in the peripheral gap having a preselected strength, whereby application of a predetermined force on the moveable member will shear the sealing material and allow the moveable member to move.