Medium for lyophilization of erythrocytes

• US 5,171,661 A
• Filed: 07/31/1990
• Issued: 12/15/1992
• Est. Priority Date: 05/18/1988
• Status: Expired due to Fees

• Current Assignee: COBE Laboratories Incorporated (Terumo Corporation)
• Original Assignee: Cryopharm Corp.
• Inventors: Goodrich, Raymond P. Jr., Williams, Christine M.
• Primary Examiner(s): Rosen, Sam
• Application Number:
  US07/560,157
• Time in Patent Office: 868 Days
• Field of Search: 435/1, 435/2, 424/533
• US Class Current: 435/2
• CPC Class Codes: A61K 35/18 Erythrocytes haemoglobin A61K38/42

Abstract

A process and medium are disclosed for the lyophilization of red blood cells which comprises the use of solutions including monosaccharide hexoses and pentoses, and/or biocompatible polymers to permit the reconstitution of viable red blood cells.

Claims

1. A medium for the lyophilization of erythrocytes, comprising a lyophilizing buffer solution which includes:

a monosaccharide which is present in the solution in a concentration of from about 0.5 molar up to about 4 molar, and

a polymer having a molecular weight of from about 10K to about 360K which is present in a concentration of at least 0.1 millimolar.

2. The medium of claim 1 wherein the monosaccharide is selected from the group consisting of pentoses and hexoses.

3. The medium of claim 1 wherein the monosaccharide is selected from the group consisting of xylose, glucose, ribose, mannose and fructose.

4. The medium of claim 1 wherein the polymer is selected from the group consisting of polyvinylpyrrolidone, and dextran.

5. The medium of claim 2 wherein the polymer is selected from the group consisting of polyvinylpyrrolidone and dextran.

6. The medium of claim 3 wherein the polymer is selected from the group consisting of polyvinylpyrrolidone and dextran.

7. The medium of claim 1 wherein the polymer is polyvinylpyrrolidone.

8. The medium of claim 2 wherein the polymer is polyvinylpyrrolidone.

9. The medium of claim 3 wherein the polymer is polyvinylpyrrolidone.