Cobalt(III) reagents in combination with water soluble polymers

• US 5,171,669 A
• Filed: 05/04/1987
• Issued: 12/15/1992
• Est. Priority Date: 05/04/1987
• Status: Expired due to Fees

• Current Assignee: Clinical Diagnostic Systems Inc.
• Original Assignee: Eastman Kodak Company
• Inventors: Burdick, Brent A., Zercie, Robert W.
• Primary Examiner(s): Kepplinger, Esther L.
• Assistant Examiner(s): Spiegel, Carol A.
• Application Number:
  US07/045,937
• Time in Patent Office: 2,052 Days
• Field of Search: 435/4, 435/29, 435/34, 436/84, 436/164, 436/169, 436/170, 436/903, 436/904, 422/55-57, 430/223-225, 260/396 R, 260/397.6, 556/138, 556/140, 556/146, 556/148
• US Class Current: 435/29
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 31/221 for investigating pH value
• CPC Class Codes: G01N 33/52 Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements immunological elements G01N33/54386; involving labelled immunochemicals G01N33/58; for haemoglobin or occult blood G01N33/72

Abstract

Water soluble polymers are useful in combination with cobalt(III) redox reagents. When incorporated with the polymers, the cobalt(III) reagents exhibit improved sensitivity compared to fresh solutions of the reagents. In preferred embodiments, the cobalt(III) reagents are coated on a support using the water soluble polymer as a binder. Small areas of the coated support are then cut out and placed in a buffer solution to provide a working reagent solution. The solution is particularly useful in the testing of urine for significant bacteriuria.

Claims

1. A reagent composition for the detection of an analyte in an aqueous sample which comprises:

(1) a water soluble cobalt(III) complex which is capable of being reduced to a water soluble cobalt(II) complex by said analyte and,

(2) a water soluble dye which is capable of being metallized by said cobalt(II) complex to form a water soluble cobalt(II) complex of the metallizable dye, wherein said cobalt(II) complex of the metallizable dye is capable of reacting with said cobalt(III) complex to produce a cobalt(III) complex of the metallizable dye and the cobalt(II) complex; and

(3) a water soluble polymer.

2. A reagent composition according to claim 1 wherein said water soluble polymer includes a repeating unit having a group selected from the group consisting of acrylamide, vinylpyrrolidone, acrylate and vinylalcohol.

3. A reagent composition according to claim 2 wherein said repeating unit is vinylpyrrolidone or acrylamide.

4. A reagent composition according to claim 3 wherein said water soluble polymer is poly(acrylamide-co-N-vinyl-2-pyrrolidone) (90:

10 by weight).

5. A reagent composition according to claim 1 further comprising an electron transfer agent.

6. A reagent composition according to claim 5 wherein said electron transfer agent is a quinone.

7. A reagent composition according to claim 1 comprising

(1) [Co(ethylenediamine)₂ (2,2'"'"'bipyridine)]Cl₃.

(2) 2-[(5-carboxy-2-pyridyl)azo]-1-naphthol-4-sulfonic acid, diammonium salt

(3) poly(acrylamide-co-N-vinyl-2-pyrrolidone) (90:

10 by weight).

8. An element comprising a support having coated thereon a reagent composition according to claim 1.

9. An element according to claim 8 wherein the water soluble dye is coated on one side of the support and the remainder of the reagents are coated on the other side of the support.

10. A method for the determination of an analyte in a liquid comprising the steps of:

(A) contacting a sample of said liquid with a reagent composition comprising;

(1) a water soluble cobalt(III) complex which is capable of being reduced to a water soluble cobalt(II) complex by said analyte and,

(2) a water soluble dye which is capable of being metallized by said cobalt(II) complex to form a water soluble cobalt(II) complex of the metallizable dye, wherein said cobalt(II) complex of the metallizable dye is capable of reacting with said cobalt(III) complex to produce a cobalt(III) complex of the metallizable dye and the cobalt(II) complex; and

(3) a water soluble polymer so as to produce a detectable change in the presence of said analyte and

(B) detecting said detectable change and relating said change to the amount of analyte in said liquid.