Solid state bio-sensor
First Claim
1. A bio-sensor for measuring a substrate concentration of a liquid sample, comprising:
- at least a working electrode and a counter electrode,an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, anda porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
1 Assignment
0 Petitions
Accused Products
Abstract
As the conventional simple bio-sensors for measuring the particular component in living bodies, there are those in which a change in dye caused by enzyme reaction is detected optically, but such bio-sensors had a problem that precision is low owing to disturbance of the color of liquid samples. With bio-sensors using an enzyme electrode, the precision was high, but operation of measurement was troublesome. The present invention made it possible to detect the concentration of the particular component electromechanically with rapidity, simplicity and high precision by merely putting a porous substrate containing at least enzyme on the electrode system and impregnating the body with a liquid sample.
-
Citations
17 Claims
-
1. A bio-sensor for measuring a substrate concentration of a liquid sample, comprising:
-
at least a working electrode and a counter electrode, an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, and a porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
-
-
2. The bio-sensor according to claim 1, wherein the buffer is a phosphate.
-
3. The bio-sensor according to claim 1, wherein an electron acceptor is carried on the porous substrate in a dry state.
-
4. The bio-sensor according to claim 1 further comprises an oxidizing dye.
-
5. The bio-sensor according to claim 4, wherein the oxidizing dye is a member selected from the group consisting of potassium ferricyanide, p-benzoquinone, 2,6-dichlorophenolindophenol, methylene blue, phenazine methosulfate and potassium beta-naphtoquinone-4-sulfonate.
-
6. The bio-sensor according to claim 1, wherein the oxidoreductase is a member selected from the group consisting of glucose oxidase, alcohol oxidase, xanthine oxidase and cholesterol oxidase.
-
7. The bio-sensor according to claim 1, wherein the oxidoreductase is glucose oxidase.
-
8. The bio-sensor according to claim 1, wherein the working electrode comprises platinum.
-
9. The bio-sensor according to claim 8, wherein the porous plate has a pore diameter of less than 3 micron meters.
-
10. The bio-sensor according to claim 1, further comprises a reference electrode.
-
11. The bio-sensor according to claim 1, wherein the porous plate is a hydrophilic porous membrane.
-
12. The bio-sensor according to claim 1, further comprising a filter having a pore size of less than 3 micron meters positioned and arranged on the porous plate.
-
13. The bio-sensor according to claim 12, wherein an anticoagulant is carried on the filter.
-
14. The bio-sensor according to claim 12, which further comprises a reference electrode.
-
15. The bio-sensor according to claim 1, wherein the counter electrode comprises platinum or silver/silver chloride.
-
16. The bio-sensor according to claim 1, wherein the pore size of the porous plate is between 1000 Angstroms and 1 mm.
-
17. A bio-sensor for measuring a substrate concentration of a liquid sample, which comprises:
-
at least a working electrode and a counter electrode, an insulating substrate plate which supports and insulates the working electrode and the counter electrode from one another, and a porous plate fixed on the insulating substrate plate, wherein said porous plate carries at least an oxidoreductase and a buffer, the oxidoreductase and the buffer are in a dry state and contacts the electrodes without an intervening electrolyte layer between (i) the oxidoreductase and the buffer and (ii) the electrodes, and wherein the bio-sensor is constructed so as not to contain a liquid containing chamber.
-
Specification