Process for using a measuring cell assembly for glucose determination

• US 5,174,291 A
• Filed: 05/15/1990
• Issued: 12/29/1992
• Est. Priority Date: 10/05/1987
• Status: Expired due to Term

• Current Assignee: Rijksuniversiteit Groningen
• Original Assignee: Rijksuniversiteit Groningen
• Inventors: Schmidt, Franciscus J., Schoonen, Adelbert J. M.
• Primary Examiner(s): Shay, David M.
• Application Number:
  US07/474,794
• Time in Patent Office: 959 Days
• Field of Search: 128/632, 128/635, 128/637, 128/760, 128/762, 128/767, 128/771, 128/DIG. 13, 604/29, 604/82, 604/83, 435/14
• US Class Current: 600/322
• CPC Class Codes: A61B 5/14528 invasively
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61B 5/686 Permanently implanted devices, e.g. pacemakers, other stimulators, biochips A61B5/6861 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

A wearable-type glucose sensor for continuously or intermittently determining the glucose content comprises a short hollow fiber (2) to be positioned in the subcutaneous tissue. This hollow fiber is connected via tubes (3,4) with component parts (5 . . . 12) located outside the body, such as a measuring unit (11). When a perfusion fluid containing the enzyme glucose oxidase is passed through the hollow fiber, a subcutaneous dialysis will take place in which some glucose dissolves in the perfusion fluid through the wall of the hollow fiber. This glucose is completely oxidized by the oxygen dissolved in the perfusion fluid in the presence of the glucose oxidase. By means of the measuring unit the resultant amount of H₂ O₂ or, preferably, the employed amount of O₂ is determined, both of which are a measure of the subcutaneous glucose concentration.

Claims

1. A process for continuously or intermittently determining the glucose concentration in subcutaneous tissue, which comprises using an enzymatic oxidation of glucose by oxygen in the presence of the enzyme glucose oxidase and determining the used amount of oxygen by means of a measuring cell, characterized in that a perfusion fluid which contains dissolved glucose oxidase, is passed continuously or intermittently at a constant rate via a supply tube through a hollow fiber provided in the subcutaneous tissue and permeable to glucose and is passed via an airtight discharge tube from the hollow fiber to a measuring cell provided outside the body, with a dialysis taking place subcutaneously whereby glucose passes via the wall of the hollow fiber from the tissue into the perfusion fluid in an amount proportional to the locally prevailing glucose concentration, and with the glucose received in the perfusion fluid being completely oxidized before reaching the measuring cell.

2. A process as claimed in claim 1 characterized in that the perfusion fluid is passed at a flow rate of 0.1-1.0 ml/hour.

3. A process as claimed in claim 1 characterized in that the perfusion fluid employed is a physiological saline solution containing the enzymes glucose oxidase and catalase in the dissolved state, the perfusion fluid is returned after passing through the measuring cell to the hollow fiber via a system comprising at least one air-permeable part, and before or after passing through the measuring cell the perfusion fluid is passed through an enzyme metering device provided outside the body, in which device a new amount of glucose oxidase and catalase is dissolved in the perfusion fluid.

4. A process as claimed in claim 3, characterized in that after passing through the measuring cell the perfusion fluid is returned to the hollow fiber via an air-permeable supply tube.