Animal model for benign prostatic disease

• US 5,175,383 A
• Filed: 02/17/1989
• Issued: 12/29/1992
• Est. Priority Date: 02/17/1989
• Status: Expired due to Term

• Current Assignee: Harvard University
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Muller, William J., Leder, Philip
• Primary Examiner(s): Weimar, Elizabeth C.
• Assistant Examiner(s): Chambers, Jasemine C.
• Application Number:
  US07/312,641
• Time in Patent Office: 1,411 Days
• Field of Search: 800/2, 800/DIG. 1, 435/172.3, 435/317.1, 935/32, 935/111
• US Class Current: 800/10
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2217/20 Animal model comprising regulated expression system
• CPC Class Codes: A01K 2227/105 Murine
• CPC Class Codes: A01K 2267/0331 Animal model for proliferative diseases
• CPC Class Codes: A01K 67/0275 Genetically modified vertebrates, e.g. transgenic
• CPC Class Codes: C07K 14/50 Fibroblast growth factor [FGF]
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic

Abstract

A male transgenic mouse containing germ cells and somatic cells which contain a recombinant gene which is a vertebrate gene in the int-2/FGF family which is capable of promoting benign prostatic hyperplasia or hypertrophy in said transgenic mouse, said gene being introduced into the mouse, or an ancestor of the mouse, at an embryonic stage.

Claims

1. A male transgenic mouse, whose germ cells and somatic cells contain an int-2 gene which is operably linked to a promoter effective for the expression of said gene in the urogenital tissues of said mouse and effective for the promotion of benign prostatic hyperplasia or hypertrophy in said mouse, said gene being introduced into said mouse, or an ancestor of said mouse, at an embryonic stage.

2. The mouse of claim 1, wherein a chromosome of said mouse includes an endogenous coding sequence substantially the same as the coding sequence of said int-2 gene.

3. The mouse of claim 2, wherein said int-2 gene has integrated into a chromosome of said mouse at a site different from the location of said endogenous coding sequence.

4. The mouse of claim 2, wherein transcription of said int-2 gene is under the control of a promoter sequence different from the promoter sequence controlling the transcription of said endogenous coding sequence.

5. The mouse of claim 4, wherein said promoter sequence controlling transcription of said int-2 gene is inducible.

6. The mouse of claim 1, wherein transcription of said int-2 gene is under the control of a viral promoter sequence.

7. The mouse of claim 6, wherein said viral promoter sequence comprises the sequence of the MMTV promoter.