Apparatus for repeated automatic execution of a thermal cycle for treatment of samples

• US 5,176,203 A
• Filed: 07/31/1990
• Issued: 01/05/1993
• Est. Priority Date: 08/05/1989
• Status: Expired due to Term

• Current Assignee: Societe de Conseils de Recherches et d'Applications Scientifiques
• Original Assignee: Societe de Conseils de Recherches et d'Applications Scientifiques
• Inventors: Larzul, M. Daniel
• Primary Examiner(s): Ford, John K.
• Assistant Examiner(s): LEO, LEONARD R
• Application Number:
  US07/560,107
• Time in Patent Office: 889 Days
• Field of Search: 435/288, 435/290, 435/316, 436/173, 436/147, 436/157, 165/61, 165/63, 165/64, 165/65, 165/120, 417/50, 73/863.11, 422/198, 422/199
• US Class Current: 165/61
• CPC Class Codes: B01L 2300/088 Channel loops
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/525 with physical movement of samples between temperature zones
• CPC Class Codes: G01N 2035/00435 Refrigerated reagent storage
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

Apparatus for repeated automatic execution of a thermal cycle for the treatment of a sample, especially a biological sample, comprises means (6) defining a pathway which is physically closed throughout the treatment and within which the sample is resident throughout the treatment. Means (3, 4, 11, 12) are provided to move the sample between different positions along the pathway and (7, 15, 16) to heat or cool the sample as a function of its position within the pathway. Preferably the pathway is a capillary tube, which may be in spiral, closed loop or linear form. Heating and cooling means are provided by thermostated zones which may provide a continuous thermal gradient regular or irregular, or be in a discontinuous arrangement. The preferred means of moving the samples is magnetic.

Claims

1. Apparatus for the treatment of biological and microbiological samples comprising:

(a) a pathway which is physically closed throughout the treatment;

(b) means for introducing one of said biological or microbiological samples to the pathway prior to the commencement of treatment and for removing said sample subsequent to the completion of treatment;

(c) means for physically closing the pathway during treatment so that nothing can be added thereto or withdrawn therefrom;

(d) a plurality of thermal means positioned along the pathway for heating or cooling of said sample, said thermal means providing treatment of said sample at a temperature of from 4°

C. to 150°

C.; and

(e) means for automatically and repeatedly, through a plurality of cycles, moving said sample between said different thermal positions along the pathway.

2. Apparatus according to claim 1 in which the pathway comprises a capillary tube.

3. Apparatus according to claim 2 in which the capillary tube is of semi-rigid plastics material.

4. Apparatus according to claim 2 in which the capillary tube has an internal diameter of from 0.1 to 4 mm.

5. Apparatus according to claim 1 in which the pathway is a spiral pathway.

6. Apparatus according to claim 1 in which the pathway is a loop.

7. Apparatus according to claim 1 in which the pathway is a linear pathway.

8. Apparatus according to claim 1 in which the means for heating or cooling the sample comprises two or more thermostated zones through which the pathway passes.

9. Apparatus according to claim 8 in which the thermostated zones are arranged discontinuously, each being separated from the next by a physical barrier crossed only by the pathway and each being provided with an autonomous heating or cooling system.

10. Apparatus according to claim 8 in which the thermostated zones are arranged continuously along the pathway to provide a continuous and directional thermal gradient.

11. Apparatus according to claim 10 in which the thermal gradient is irregular.

12. Apparatus according to claim 1 in which the means for moving the sample comprises magnetic means including magnetic party within the pathway adjacent to the sample and an external magnet acting on the magnetic party to move it and hence the sample.

13. Apparatus according to claim 12 in which the magnetic party is a solid magnet or a suspension of magnetic microparticles in a liquid immiscible with the sample.

14. Apparatus according to claim 1 in which the means for moving the sample comprises a pump acting on a gas.

15. Apparatus according to claim 1 in which the means for moving the sample comprises the effect of thermal pumping created by the proximity of gaseous masses at different temperatures within the pathway.

16. Apparatus according to claim 2 in which the means for moving the sample comprises passive capillary action.

17. Apparatus according to claim 1 in which the means for moving the sample comprises a peristaltic pump.

18. Apparatus for the treatment of biological and microbiological samples comprising:

(a) a loop pathway which is physically closed throughout the treatment;

(b) means for introducing one of said biological or microbiological samples to the loop pathway prior to the commencement of treatment and for removing said sample subsequent to the completion of treatment;

(c) means for physically closing the loop pathway during treatment so that nothing can be added thereto or withdrawn therefrom;

(d) a plurality of thermal means positioned along the loop pathway for heating or cooling of said sample, said thermal means providing treatment of said sample at a temperature of from 4°

C. to 150°

C.; and

(e) means for automatically and repeatedly, through a plurality of cycles, moving said sample between said different thermal positions along the loop pathway.