Biosensor containing immobilized Zymomonas mobilis cells for measuring glucose, fructose and sucrose

• US 5,177,012 A
• Filed: 08/27/1990
• Issued: 01/05/1993
• Est. Priority Date: 10/25/1989
• Status: Expired due to Fees

• Current Assignee: Korea Advanced Institute of Science and Technology
• Original Assignee: Korea Advanced Institute of Science and Technology
• Inventors: Park, Je-Kyun, Kim, Hak-Sung
• Primary Examiner(s): Naff, David M.
• Application Number:
  US07/572,254
• Time in Patent Office: 862 Days
• Field of Search: 435/14, 435/174, 435/175, 435/, 435/177, 435/178, 435/180, 435/181, 435/182, 435/817, 204/403
• US Class Current: 204/403.1
• CPC Class Codes: C12N 11/02 Enzymes or microbial cells immobilised on or in an organic carrier
• CPC Class Codes: C12N 11/04 entrapped within the carrier, e.g. gel or hollow fibres
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A biosensor for the determination of glucose and fructose concentrations is provided. The biosensor is produced by a method which comprises treating Zymomonas mobilis whole cells with an organic solvent such as xylene and n-butanol, immobilizing the treated whole cells onto a support selected from the group consisting of gelatin, collagen, agarose, cellophane and polyacrylamide to give an immobilized whole cell enzyme membrane and adhering the membrane to the surface of a pH electrode to give the biosensor. The resulting biosensor is capable of determning glucose and fructose in high concentrations such as 5 g/L and 50 g/L, respectively. Invertase can be immobilized with the whole cells to provide a biosenser for the determination of sucrose.

Claims

1. A process for the production of a biosensor for measuring glucose and fructose which comprises treating Zymomonas mobilis whole cells with an organic solvent, immobilizing the treated whole cells onto a support selected from the group consisting of gelatin, collagen, agarose, cellophane and polyacrylamide to give an immobilized whole cell enzyme membrane, and adhering the membrane to the surface of a pH electrode to give the biosensor.

2. The process of claim 1, in which said organic solvent is xylene or n-butanol.

3. The process of claim 1, in which an invertase is further immobilized concurrently with the whole cells treated with the organic solvent.

4. The process of claim 3, in which the biosensor produced is used for the determination of sucrose concentration.

5. A biosensor for measuring glucose and fructose comprising:

a) an immobilized whole cell enzyme membrane; and

b) a pH electrode,

said membrane being adhered to the surface of said pH electrode and comprising Zymomonas mobilis whole cells which have been permeabilized with an organic solvent and then immobilized onto a support selected from the group consisting of gelatin, collagen, agarose, cellophane and polyacrylamide.

6. The biosensor of claim 5, wherein said membrane further comprises an invertase co-immobilized with the whole cells.

7. The biosensor of claim 6, wherein said biosensor is a biosensor for measuring sucrose concentration.