Soybean peroxidase treatment of contaminated substances

• US 5,178,762 A
• Filed: 04/29/1992
• Issued: 01/12/1993
• Est. Priority Date: 10/18/1990
• Status: Expired due to Fees

• Current Assignee: Wiley Organics Inc.
• Original Assignee: MEAD Corporation (Meadwestvaco Corporation)
• Inventors: Johnson, Mark A., Pokora, Alexander R.
• Primary Examiner(s): Bascomb, Jr., Wilbur
• Assistant Examiner(s): MCCARTHY, NEIL
• Application Number:
  US07/876,509
• Time in Patent Office: 258 Days
• Field of Search: 210/721, 210/728, 210/759, 210/912-914, 210/928, 210/908, 210/909, 210/917, 210/903, 210/632, 435/192, 435/262, 435/264, 435/41, 435/28, 162/29, 162/72 B, 162/190
• US Class Current: 210/632
• CPC Class Codes: B09C 1/08 chemically
• CPC Class Codes: B09C 1/10 microbiologically, biologically or by using enzymes
• CPC Class Codes: C02F 3/342 characterised by the enzymes used
• CPC Class Codes: C08G 61/00 Macromolecular compounds obtained by reactions forming a carbon-to-carbon link in the main chain of the macromolecule C08G2/00 - C08G16/00 take precedence
• CPC Class Codes: C12N 9/0065 acting on hydrogen peroxide as acceptor (1.11)
• CPC Class Codes: C12P 7/22 aromatic
• CPC Class Codes: Y10S 210/908 Organic
• CPC Class Codes: Y10S 210/909 Aromatic compound, e.g. pcb, phenol
• CPC Class Codes: Y10S 210/912 Heavy metal
• CPC Class Codes: Y10S 210/917 Color
• CPC Class Codes: Y10S 210/928 Paper mill waste, e.g. white water, black liquor treated

Abstract

A method for oxidizing organic and/or heavy metal contaminants in wastewaters, sludges, or soils containing such contaminants by contacting the wastewaters, sludges, or soils with soybean peroxidase and a peroxide.

Claims

1. A method for oxidizing organic and/or heavy metal contaminants in wastewaters, sludges, or soils containing said contaminants which comprises contacting said wastewaters, sludges or soils with soybean peroxidase and a peroxide.

2. The method of claim 1 wherein said wastewaters, sludges or soils are contacted with ground soybean hulls.

3. The method of claim 1 wherein said peroxide is hydrogen peroxide.

4. The method of claim 3 wherein said method is for treating wastewater.

5. The method of claim 4 wherein said wastewater is contacted with about 0.05 to 5 units of soybean peroxidase per liter of wastewater.

6. The method of claim 5 wherein said wastewaters are treated with about 0.1 to 10 mmoles hydrogen peroxide per liter of said wastewaters, said treatment conducted at a temperature up to about 60°

C. and at a pH of about 3 to 10.

7. The method of claim 6 wherein said treatment reduces the amount of AOX in said wastewater.

8. The method of claim 6 wherein said treatment reduces the amount of phenols and aromatic amines in said wastewater.

9. The method of claim 6 wherein said treatment reduces the amount of color bodies, heavy metals, or mixtures thereof.

10. The method of claim 9 wherein said wastewater is pulp mill effluents.

11. The method of claim 6 wherein said treatment oxidizes heavy metals and renders them more readily removable.

12. The method of claim 3 wherein said method is for treating soils or sludges.

13. The method of claim 12 wherein said soils or sludges are mixed with soybean peroxidase and a solution of hydrogen peroxide.

14. The method of claim 13 wherein said soybean peroxidase is mixed as ground soybean hulls.

15. The method of claim 13 wherein said method converts phenols or aromatic amines to less hazardous polymeric phenols or amines.

16. The method of claim 11 wherein said treatment includes the additional step of removing said heavy metals.

17. The method of claim 16 wherein said heavy metals are removed by ion exchange or chelation.

18. The method of claim 6 wherein said hydrogen peroxide is added under conditions such that said peroxide does not substantially inhibit the reaction of said peroxidase.