Automatic immunoassay analyzer

• US 5,178,834 A
• Filed: 06/03/1992
• Issued: 01/12/1993
• Est. Priority Date: 07/19/1989
• Status: Expired due to Term

• Current Assignee: Tosoh Corporation
• Original Assignee: Tosoh Corporation
• Inventors: Iwasaki, Shuji, Kagayama, Toshi
• Primary Examiner(s): Housel, James C.
• Assistant Examiner(s): Le, Long V.
• Application Number:
  US07/892,486
• Time in Patent Office: 223 Days
• Field of Search: 422/63, 422/65, 422/67, 422/68.1, 422/107, 422/73, 436/43, 436/47, 436/50, 436/54, 436/526
• US Class Current: 422/65
• CPC Class Codes: G01N 2035/00376 Conductive heating, e.g. heated plates
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/0408 connected in a flexible chain
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 2035/1086 Cylindrical, e.g. variable angle
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/021 having a flexible chain, e.g. "cartridge belt", conveyor for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1004 Cleaning sample transfer devices
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack

Abstract

An automatic immunoassay analyzer includes first and second conveyors which support a plurality of speciment vessels and reaction vessels, respectively. Both conveyors are rotated synchronously and continuously in the same direction by first and second conveyor driving devices. A division-pouring device sucks a speciment from a speciemen vessel stopped at a suction stage and pours a portion thereof into a reaction vessel stopped at a division-pouring stage. A holding device stops and holds one of the specimen vessels transferred into the suction stage for a predetermined time period in which the reaction vessel(s) holding measurement analytes to be tested for the respective specimen are transferred and stopped sequentially at the division-pouring stage. The analyzer reduces operator error, and ensures alignment of the reaction vessels with the appropriate specimen to be tested.

Claims

1. An automatic immunoassay analyzer, comprising:

an endless specimen vessel transfer conveyor having a plurality of support portions for supporting a plurality of specimen vessels, said specimen vessel transfer conveyor conveying said plurality of support portions in a first direction;

a reaction vessel transfer conveyor having a plurality of support portions for supporting a plurality of reaction vessels, said reaction vessel transfer conveyor conveying said plurality of support portions in said first direction;

division-pouring means for sucking a specimen from a specimen vessel stopped at a suction stage and pouring a portion thereof into a reaction vessel stopped at a division-pouring stage, said specimen vessel transfer conveyor including an endless transport path thereof from a first set stage at which said specimen vessels fitted into and supported by said support portions, and returning to said first set stage through said suction stage at which said specimen inside each of said specimen vessels being sucked by said division-pouring means;

first conveyor driving means for sequentially conveying and stopping said specimen vessels to and at said suction stage corresponding to a respective support portion zone determined sequentially by zoning said support portions of said specimen vessel transfer conveyor by a number corresponding to a number of measurement analytes of each of said specimens supported;

said reaction vessel transfer conveyor including a second endless transport path thereof from a second set stage at which said reaction vessels fitted into and supported by said support portions, through said division-pouring stage at which a portion of the specimen sucked from a respective specimen vessel being poured into said reaction vessel by said division-pouring means, through a reaction-measurement stage at which a measurement of an immunological reaction inside said reaction vessels being performed, and returning to said second set stage;

second conveyor driving means for sequentially transferring, stopping and feeding out said reaction vessels to, at and from said division-pouring stage when said reaction vessels corresponding to said measurement analytes of each of said specimen being sequentially supported by said support portions of said reaction vessel transfer conveyor; and

means for synchronously and simultaneously actuating said first and second conveyor driving means;

holding means downstream of said first conveyor driving means for stopping and holding one of said specimen vessels transferred into said suction stage for a predetermined time period independent of said first conveyor driving means in which said reaction vessels corresponding to said specimen being transferred and stopped sequentially into and at said division-pouring stage,

means for accommodating excess specimen vessel transfer conveyor and specimen vessels between said holding means and said first conveyor driving means as a result of any difference in said time period and said actuation of said first conveyor driving means.

2. An automatic immunoassay analyzer according to claim 1, wherein at least one of said specimen vessel transfer conveyor and said reaction vessel transfer conveyor is a conveyor capable of zigzag movement inside a horizontal plane.

3. An automatic immunoassay analyzer according to claim 2, wherein said conveyor capable of zigzag movement is an endless chain conveyor.

4. An automatic immunoassay analyzer according to claim 2, wherein at least one of said first conveyor driving means and said second conveyor driving means for rotating the respective one of said specimen vessel transfer conveyor and said reaction vessel transfer conveyor capable of zigzag movement is a sprocket rotating intermittently while engaging the respective conveyor.

5. An automatic immunoassay analyzer according to claim 4, wherein said sprockets of said first and second conveyor driving means rotate synchronously.

6. An automatic immunoassay analyzer according to claim 2, wherein said first conveyor driving means for rotating said specimen vessel transfer conveyor comprises a first sprocket disposed at said suction stage for transferring, stopping and feeding out said specimen vessel into, at and from said suction stage and a plurality of second sprockets for feeding intermittently said support portions one frame at a time, disposed at positions other than at said suction stage.

7. An automatic immunoassay analyzer according to claim 6, further comprising first and second stay regions, said first and second stay regions each comprising an area at which said specimen vessel transfer conveyor fed one frame at a time by said plurality of second sprockets remains in a zig-zag form, said first and second stay regions being disposed upstream and downstream of said suction stage, respectively.

8. An automatic immunoassay analyzer according to claim 7, wherein said holding means stops said first sprocket for a stop period corresponding to a number of measurement analytes of each of said specimens, and rotates continuously said first sprocket at a same timing as a next one-frame feed operation of said plurality of second sprockets after said stop period until a next one of said specimen vessels on said conveyor staying in the zig-zag form of said first stay region being transferred into said suction stage.

9. An automatic immunoassay analyzer according to claim 1, wherein said holding means moves said specimen vessels transferred to said suction stage from said specimen vessel transfer conveyor in a predetermined direction, said predetermined direction being transverse to said first direction of said specimen vessel transfer conveyor.

10. An automatic immunoassay analyzer according to claim 8, further comprising counter means for detecting the number of measurement analytes of each of said specimen vessels being transferred by said specimen vessel transfer conveyor, said counter means being disposed at a position upstream of said suction stage of said specimen vessel transfer conveyor, and control means for driving and controlling said first sprocket to advance said conveyor a predetermined distance based on the number of measurement analytes detected by said counter means.

11. An automatic immunoassay analyzer according to claim 10, wherein said counter means includes means for detecting said specimen vessel supported by a leading one of said support portions of each of said support portion zones, and means for detecting the number of the measurement analytes by counting empty support portions not supporting thereon subsequent specimen vessels.

12. An automatic immunoassay analyzer according to claim 1, wherein said support portions of both of said specimen vessel transfer conveyor and said reaction vessel transfer conveyor are disposed equidistantly in said first direction.

13. An automatic immunoassay analyzer according to claim 1, wherein said first transport path of said specimen vessel transfer conveyor in said first set stage and said second transport path of said reaction vessel transfer conveyor in said second set stage are disposed in parallel with each other.

14. An automatic immunoassay analyzer according to claim 1, further comprising first read means for reading a mark specifying said specimen displayed on said specimen vessel disposed at a position upstream of said suction stage of said specimen vessel transfer conveyor in said first direction.

15. An automatic immunoassay analyzer according to claim 1 further comprising second read means for reading measurement analytes displayed on said reaction vessel disposed at a position upstream of said division-pouring stage of said reaction vessel transfer conveyor.

16. An automatic immunoassay analyzer according to claim 1, wherein said division-pouring means comprises a pipet device moving between said suction stage of said specimen vessel transfer conveyor and said division-pouring stage of said reaction vessel transfer conveyor.

17. An automatic immunoassay analyzer according to claim 1, wherein an antibody causing the immunological reaction with an antigen contained in said specimen is prepacked into said reaction vessel.

18. An automatic immunoassay analyzer according to claim 17, wherein the antibody is fixed to a solid phase.

19. An automatic immunoassay analyzer according to claim 1, wherein said reaction-measurement stage to which said reaction vessel conveyed comprises reagent division-pouring means for charging a portion of a reagent containing an antibody causing the immunological reaction with an antigen contained in said specimen, into said reaction vessel.

20. An automatic immunoassay analyzer according to claim 19, wherein said antibody contained in said reagent being labeled with an enzyme, and said reaction-measurement stage includes substrate division-pouring means for charging a portion of a substrate into said reaction vessel causing an optically detectable change by activity of said enzyme and optical detection means for detecting a change in said substrate as a result of said immunological reaction.

21. An automatic immunoassay analyzer according to claim 20, further comprising recording means for recording the detection result by said optical detection means.

22. An automatic immunoassay analyzer according to claim 21, further comprising first read means for reading a mark specifying said specimen which is displayed on said specimen vessel disposed at a position upstream of said suction stage of said specimen vessel transfer conveyor in said first direction, and

second read means for reading the measurement analytes displayed on said reaction vessel disposed at a position upstream of said division-pouring stage of said reaction vessel transfer conveyor,

wherein said recording means records a read result of said first read means and a read result of said second read means with a measurement result of said optical detection means.

23. An automatic immunoassay analyzer according to claim 1, further comprising a solid having magnetic susceptibility prepacked into said reaction vessel, and means for imparting an oscillating magnetic field on said reaction vessel disposed on said reaction-measurement stage.

24. An automatic immunoassay analyzer according to claim 18, further comprising a solid having magnetic susceptibility pre-packed into said reaction vessel, and means for imparting an oscillating magnetic field on said reaction vessel disposed on said reaction-measurement stage,

wherein said solid pre-packed into said reaction vessel comprises said solid phase.

25. An automatic immunoassay analyzer according to claim 1, wherein support portion zones being sequentially zoned on said specimen vessel transfer conveyor by the number of measurement analytes of said specimen, and a corresponding one of said specimen vessels being supported by a leading support portion of each of said support portion zones.

26. An automatic immunoassay analyzer according to claim 19, further comprising means for incubating said reaction vessel containing said reagent and said specimen and being positioned at said reaction-measurement stage to which said reaction vessel being transferred, said incubating means comprising a heat generator bonded to a lower surface of a sheet forming said second transport path, wherein current flowing through said heat generator to control the temperature of said reaction-measurement stage to a predetermined temperature.

27. An automatic immunoassay analyzer according to claim 8, further comprising a vessel sensor disposed upstream of said suction stage for detecting a specimen vessel, and a reciprocating cylinder disposed on said suction stage,

wherein upon detection of one of said specimen vessels, said reciprocating cylinder being driven in synchronism with the next intermittent rotation of the said specimen vessel transfer conveyor,

said reciprocating cylinder including a plunger movable in first and second directions opposite to one another across said specimen vessel transfer conveyor from a first side thereof to a second side thereof in a direction orthogonal to said first direction of said specimen vessel transfer conveyor, and a return mechanism disposed on said second side of said specimen vessel transfer conveyor to oppose said plunger,

wherein said plunger being engageable with said specimen vessel detected by said vessel sensor to push the specimen vessel outwardly from said specimen vessel transfer conveyor, and said return mechanism comprises a pad and a return spring for biasing said pad towards said specimen vessel transfer conveyor.