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Minimum procedure system for the determination of analytes

  • US 5,179,005 A
  • Filed: 04/28/1988
  • Issued: 01/12/1993
  • Est. Priority Date: 08/13/1986
  • Status: Expired due to Term
First Claim
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1. A method for determining glucose in a blood sample employing a membrane and a signal-producing system which reacts with glucose to produce a light-absorptive dye product, said system being bound to the membrane, and in which the amount of said dye product is determined by means of a reflectance measurement from a surface of said membrane, an improvement which comprises:

  • applying an unmeasured whole blood sample to a first porous, hydrophilic membrane having pores of a size sufficient to exclude red blood cells and which contains said signal-producing system;

    placing said matrix in a reflectance scanning meter wherein said meter detects the presence of said blood sample on said matrix, said reflectance scanning meter initiating a timing sequence upon detecting the presence of said blood sample in matrix;

    taking reflectance measurements;

    (1) from the background within said meter (Rb);

    (2) before said matrix contains blood (Rdry); and

    (3) at a predetermined time (Rt), then computing R*t wherein
    
    
    space="preserve" listing-type="equation">R*t=(R.sub.t -R.sub.b)/(R.sub.dry -R.sub.b);

    using R*t to compute K/S-t, wherein K/S-t is defined as
    
    
    space="preserve" listing-type="equation">(1-R*t).sup.2 /(2×

    R*t); and

    computing glucose levels from said K/S-t value.

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