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Process for measuring endotoxin

  • US 5,179,006 A
  • Filed: 02/23/1989
  • Issued: 01/12/1993
  • Est. Priority Date: 02/27/1988
  • Status: Expired due to Term
First Claim
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1. In a process for measuring an amount of endotoxin in a sample containing an unknown amount of endotoxin by using a reaction of a horseshoe crab hemocyte lysate with endotoxin in a solution, the improvement which comprises carrying out the reaction in the presence of at least one water-soluble polysaccharide selected from the group consisting of curdlan, pachyman, scleratan, lentinan, schizophyllan, coriolan, laminaran, laminarin, paramilon and carboxyne thylated curdlan, in an mount sufficient to inactivate a factor present in the AL solution which reacts with beta-1,3-glucan, and determining the concentration of endotoxin in said unknown sample.

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