Apparatus for and method of fractionating particle in particle-suspended liquid in conformity with the properties thereof

• US 5,180,065 A
• Filed: 10/11/1990
• Issued: 01/19/1993
• Est. Priority Date: 10/11/1989
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Yoneyama, Yoshito, Touge, Yoshiyuki
• Primary Examiner(s): Hajec, Donald T.
• Application Number:
  US07/596,083
• Time in Patent Office: 831 Days
• Field of Search: 209/3.1, 209/576-577, 209/579, 209/639, 209/644, 209/906, 209/587, 222/420, 356/39, 417/52, 346/75
• US Class Current: 209/577
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1409 Handling samples, e.g. injecting samples
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/0238 Single particle scatter
• CPC Class Codes: Y10S 209/906 Pneumatic or liquid stream feeding item

Abstract

This invention measures the properties of individual particles in particle-suspended liquid by an optical technique or the like, causes the particle-suspended liquid to fall while separating the liquid into droplets containing the individual particles therein, and causes liquid droplets discharged from a nozzle to collide with the droplets from a direction differing from the direction of fall, in conformity with the properties of the particles discriminated by the measurement, so as to change the direction of fall of the falling droplets, thereby fractionating the particles.

Claims

1. An apparatus for fractionating particles in particle-suspended liquid in conformity with properties thereof, comprising:

measuring means for measuring the properties of individual particles in the particle-suspended liquid;

separating means for separating said particle-suspended liquid into separated droplets containing a plurality of the individual particles therein; and

discharging means including a nozzle for selectively discharging a liquid droplet from said nozzle to collide with said separated droplets in accordance with a discharge signal generated on the basis of the result of said measurement by said measuring means.

2. An apparatus according to claim 1, wherein measuring means comprises optical measuring means for optically measuring the individual particles.

3. An apparatus according to claim 2, wherein said optical measuring means has:

a light source;

means for applying a light from said light source to the individual particles; and

means for detecting the light from the particles to which the light is applied.

4. An apparatus according to claim 3, wherein said means for detecting the light has a sensor for detecting scattered light and/or fluorescence from the particles.

5. An apparatus according to claim 1, wherein said separating means forms the particle-suspended liquid into a fine stream and injects it into an atmosphere, and imparts vibration to it to thereby form it into droplets.

6. An apparatus according to claim 1, wherein said separating means discharges a slight amount of said particle-suspended liquid from a nozzle to thereby form it into droplets.

7. An apparatus according to claim 1, wherein said particles comprise cells.

8. An apparatus according to claim 1, further comprising accumulating means for discretely accumulating the particles fractionated in conformity with the properties thereof.

9. An apparatus according to claim 1, wherein said discharged droplet comprises physiological saline solution.

10. An apparatus according to claim 1, wherein said nozzle has a heating member, and said heating member is heated in accordance with said discharge signal to thereby create a bubble in the liquid in said nozzle and discharge droplets thereof.

11. An apparatus according to claim 1, wherein said nozzle has a vibration member and wherein said vibration member is vibrated in accordance with said discharge signal to thereby impart to the liquid in said nozzle and discharge droplets thereof.

12. An apparatus for fractionating particles in particle-suspended liquid in conformity with the properties thereof, comprising:

means for causing individual particles in the particle-suspended liquid to flow to a position to be examined;

measuring means for measuring the properties of the individual particles flowing at the position to be examined;

separating means for separating said particle-suspended liquid into droplets containing the individual particles therein; and

discharging means including a nozzle for selectively discharging a liquid droplet from said nozzle to collide with said separated droplets in accordance with a discharge signal generated on the basis of the result of said measurement by said measuring means.

13. An apparatus according to claim 12, further comprising accumulating means for discretely accumulating the particles fractionated in conformity with the properties thereof.

14. An apparatus according to claim 12, wherein said particles comprise cells.

15. A method of fractionating particles in a particle-suspended liquid in conformity with the properties thereof, comprising the steps of:

measuring the properties of individual particles in the particle-suspended liquid;

generating a discharge signal on the basis of a result from said measuring;

separating said particle-suspended liquid into droplets containing the individual particles therein; and

selectively discharging a liquid droplet from a nozzle to collide with said separated droplets in accordance with the discharge signal generated on the basis of the result of said measuring.

16. An apparatus according to claim 15, wherein said particles comprise cells.