Liquefaction of granular-starch slurries using alpha-amylase in the presence of carbonate ion

• US 5,180,669 A
• Filed: 03/27/1991
• Issued: 01/19/1993
• Est. Priority Date: 03/27/1991
• Status: Expired due to Term

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Antrim, Richard L.
• Primary Examiner(s): Naff, David M.
• Assistant Examiner(s): Ware, Deborah K.
• Application Number:
  US07/676,770
• Time in Patent Office: 664 Days
• Field of Search: 435/836, 435/99, 435/100
• US Class Current: 435/99
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: Y10S 435/836 Bacillus licheniformis

Abstract

A starch slurry is liquified with alpha-amylase to produce fructose by a process wherein carbonate ion in excess of an amount needed to adjust the pH to about 5.0 to 6.0 is added to the slurry and the pH of the slurry is adjusted to about 5.0 to 6.0. The excess amount is preferably about 2 mM to 20 mM. This process enables obtaining consistent and complete liquefaction.

Claims

1. A process of liquefying a granular starch slurry comprising:

(1)

a) adding to the slurry an excess of carbonate ion in an amount of from about 2 mM to about 20 mM in excess of the amount of carbonate ion needed to adjust the pH of the slurry to a pH of about 5.0 to about 6.0 and adjusting the pH of the slurry to a pH from about 5.0 to about 6.0;

b) adding to the slurry about 10 liquefons or more of an alpha-amylase per gram of dry starch in the slurry; and

(2) heating the slurry from step (1) at a temperature from about 100°

C. to about 105°

C. for an initial period and then holding the slurry at a temperature from about 90°

C. to about 100°

C. for a length of time sufficient to liquefy the starch.

2. A process according to claim 1 wherein the alpha amylase is derived from Bacillus lichenformis.

3. A process according to claim 1 wherein in step (2) the initial period comprises from about 2 minutes to about 10 minutes.

4. A process according to claim 1 wherein the carbonate ion is provided by addition of sodium bicarbonate, sodium carbonate or carbon dioxide.

5. A process according to claim 1 which is performed until the DE of the slurry is about 10 or less.

6. A process of liquefying a granular starch slurry comprising:

(1)

a) adding to the slurry an excess of carbonate ion in an amount of from about 2 mM to about 20 mM in excess of the amount of carbonate ion needed to adjust the pH of the slurry to a pH of about 5.0 to about 6.0 and adjusting the pH of the slurry to a pH from about 5.0 to about 6.0;

b) adding to the slurry from about 10 liquefons to about 14 liquefons of an alpha-amylase per gram of dry starch in the slurry; and

(2) heating the slurry from step (1) for an appropriate time and at an appropriate temperature effective to liquefy the starch.

7. A process of claim 6, wherein the alpha-amylase is derived from Bacillus licheniformis.

8. A process of claim 6, wherein the slurry is heated in step (2) at a temperature from about 100°

C. to about 105°

C. for an initial period of from about 2 minutes to about 10 minutes and then holding the slurry at a temperature from about 90°

C. to about 100°

C. for a period sufficient to liquefy the starch.