Multiple aliquot device for distributing a liquid solution into a well

• US 5,182,082 A
• Filed: 01/23/1991
• Issued: 01/26/1993
• Est. Priority Date: 01/23/1991
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Monthony, James F., Livingston, Dwight, Tite, Robert C., Reuben, Jayakumar
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/644,786
• Time in Patent Office: 734 Days
• Field of Search: 435/30, 435/287, 435/292-294, 422/99-100, 422/ 102-104, 422/50, 422/61, 422/55, 422/57, 137/255, 137/571, 137/561 A, 141/35-36, 141/234, 141/236-240, 141/244
• US Class Current: 422/408
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: Y10T 137/85938 Non-valved flow dividers

Abstract

The present invention is a device which allows for receiving, distributing and storing a sample into numerous aliquots of small volume without air entrapment and with retention of aliquots when the device is manipulated. The device allows for the treatment of any or all of the aliquots with the same or different reagents and/or other chemical additives. The device comprises a housing for containing a body for guiding a sample into a plurality of wells without the need for pipetting aids, without multiple manipulations, without the retention of air and for retaining sample in the wells.

Claims

1. A device for dividing a sample into aliquots without air entrapment and for retention of aliquots therein, comprising:

a sample distribution element comprising an upper surface and a lower surface substantially parallel to each other, means for containing and/or guiding said sample associated with said upper surface, and at least one well disposed between said upper and lower surfaces wherein said well comprises an upper mouth opening that forms a substantially sharp junction with said upper surface, a lower mouth opening in said lower surface, a sidewall connecting said upper mouth opening with said lower mouth opening, and a sidewall bottom surface associated with said lower mouth opening;

and an outer-base element associated with a portion of said sample distribution element comprising a bottom with an inner surface and at least one well bottom raised from said inner surface;

wherein said sidewall bottom surface of said well of said sample distribution element and said well bottom of said outer-base element are substantially parallel to each other and form a spaced mean stop allow the escape of air.

2. The device of claim 1 further comprising:

a plurality of posts projecting from said inner surface of said outer-base element; and

a plurality of corresponding bosses associated with said lower surface of said sample distribution element,

wherein said posts and corresponding bosses engage to connect said outer-base element and said sample distribution element.

3. The device of claim 1 wherein said sidewall bottom surface of said well comprises a periphery with a projection.

4. The device of claim 1 wherein said sidewall bottom surface of said well comprises a periphery with an abraded surface.

5. The device of claim 1 wherein said well bottom of aid outer-base element comprises a periphery with a projection.

6. The device of claim 1 wherein said well bottom of said outer-base element comprises a periphery with an abraded surface.

7. The device of claim 1 wherein said means or containing and/or guiding said sample over said upper surface of said sample distribution element is a trough.

8. The device of claim 7 wherein said trough comprises a pathway on said upper surface of said sample distribution element.

9. The device of claim 7 further comprising a means or receiving, distributing and storing said sample.

10. The device of claim 9 wherein said means for receiving, distribution and storing said sample is a reservoir adjacent said trough.

11. The device of claim 1 wherein said upper mouth opening of said well of said sample distribution element is from about 0.03 cm to about 0.64 cm in diameter.

12. The device of claim 11 wherein said upper mouth opening is about 0.41 cm in diameter.

13. The device of claim 1 wherein said well of said sample distribution element is a height from about 0.1 cm to about 5 cm.

14. The device of claim 13 wherein said well of said sample distribution element is a height of about 0.41 cm.

15. The device of claim 1 wherein said sidewall of said well is coated with dried reagents.

16. The device of claim 1 further comprising a lid comprising an upper surface, a lower surface, sidewalls adjacent to said upper surface and substantially perpendicular to said lower surface, and at least one projection depending from said lower surface.

17. The device of claim 16 wherein said projection of said lid corresponds in location with and fists within said upper mouth opening of said well of said sample distribution element.

18. The device of claim 16 wherein said projection comprise reagents coated thereon.

19. The device of claim 16 further comprising at least one extension raised from said upper surface of said lid for viewing said projection.

20. The device of claim 16 further comprising:

an opening on said lid upper surface; and

a conduit on said lid lower surface connected to said opening and to said projection.

21. The device of claim 20 further comprising a removable seal affixed to said upper surface of said lid over said opening.

22. The device of claim 16 further comprising absorption means on said lid lower surface.

23. The device of claim 22 wherein said absorption means comprises a sponge or absorbent pad.

24. The device of claim 16 wherein said projection is a plurality of projections.

25. The device of claim 24 further comprising:

an opening on said lid upper surface;

a conduit on said lid lower surface connected to said opening and to said plurality of projections.

26. The device of claim 1 wherein said well is a plurality of wells.

27. The device of claim 26 wherein said sample distribution element is substantially rectangular in shape and said wells of said sample distribution element are in an ordered array of substantially parallel rows.