Monoclonal antibodies specific for non-A1C glycated hemoglobin and immunoassay methods
First Claim
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1. A hybridoma which produces an antibody which specifically binds to an epitope on glycohemoglobin comprising epsilon-D-fructosyllysine and not present on hemoglobin AO, or hemoglobin A1c or other proteins.
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Abstract
Monoclonal antibodies specific for an epitope found on hemoglobin glycated both in vivo and in vitro but not found on hemoglobin Ao or hemoglobin A1c are provided. Use of these antibodies to measure glycohemoglobin levels in the blood provides a measure of the glucose levels in the erythrocyte for the previous 90-120 days. No reduction of the blood sample is required to produce the epitope recognized by the monoclonal antibodies.
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10 Claims
- 1. A hybridoma which produces an antibody which specifically binds to an epitope on glycohemoglobin comprising epsilon-D-fructosyllysine and not present on hemoglobin AO, or hemoglobin A1c or other proteins.
- 3. A monoclonal antibody which specifically binds to an epitope on glycohemoglobin comprising epsilon-D-fructosyllysine and not present on hemoglobin Ao, or hemoglobin A1c or other proteins.
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6. A method of measuring the amount of glycohemoglobin in a blood sample, consisting essentially of the steps of:
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obtaining a sample of human blood comprising erythrocytes; lysing the erythrocytes in the sample; contacting the sample with a monoclonal antibody which specifically binds to an epitope on glycohemoglobin comprising epsilon-D-fructosyllysine and not present on hemoglobin Ao, hemoglobin A1c or other proteins, said contacting occurring under conditions which allow antibody-antigen reactions to occur; determining the amount of the monoclonal antibody bound to components of the sample, the amount of antibody bound being proportional to the amount of glycohemoglobin in the sample. - View Dependent Claims (7, 8)
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Specification