Integral multilayer element for glucose analysis

• US 5,183,741 A
• Filed: 10/22/1990
• Issued: 02/02/1993
• Est. Priority Date: 10/13/1983
• Status: Expired due to Term

• Current Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Arai, Fuminori, Katsuyama, Harumi
• Primary Examiner(s): Naff, David M.
• Application Number:
  US07/603,243
• Time in Patent Office: 834 Days
• Field of Search: 435/14, 435/25, 435/28, 435/174, 435/175, 435/176, 435/180, 435/805, 422/56, 422/57
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: Y10S 435/805 Test papers

Abstract

An integral multilayer element for chemical analysis utilizing an oxidase enzyme reaction system is prepared having in the following order:

(a) a porous spreading layer;

(b) an oxygen-permeable, protein-impermeable light-blocking layer;

(c) an oxidase-containing layer;

(d) an indicator layer containing peroxidase and a hydrogen peroxide indicator showing detectable change in the presence of peroxidase and hydrogen peroxide; and

(e) a water-impermeable, light-transmissive support. The oxidase in the oxidase-containing layer is preferably glucose oxidase to provide a multilayer element for glucose determination. This multilayer element shortens the analytical time, widens the measurable concentration range of the analyte and improves accuracy.

Claims

1. An integral multilayer element for chemical analysis of glucose in a liquid sample, which comprises, in the following order:

(a) a porous spreading layer;

(b) an oxygen-permeable, protein-impermeable light-blocking layer;

(c) a layer containing glucose oxidase;

(d) an indicator layer containing peroxidase and an indication for hydrogen peroxide showing a photometrically detectable change in the presence of peroxidase and hydrogen peroxide; and

(e) a water-impermeable, light-transmissive support.

2. The integral multilayer element of claim 1 wherein said light-blocking layer comprises a light-blocking titanium dioxide powder dispersed in a hydrophilic polymer binder, the ratio of the titanium dioxide powder to said polymer binder being within the range of from about 10:

0.6 to 10;

1.8 by weight.

3. The integral multilayer element of claim 1 wherein said porous spreading layer is made of fabric.

4. The integral multilayer element of claim 1 wherein said layer containing glucose oxidase comprises a glucose oxidase and a hydrophilic polymer.

5. The integral multilayer element of claim 1 wherein said layer containing glucose oxidase comprises a glucose oxidase, a cationic mordant, and a hydrophilic polymer.