Device and method for photoactivation

• US 5,184,020 A
• Filed: 10/26/1989
• Issued: 02/02/1993
• Est. Priority Date: 10/26/1989
• Status: Expired due to Fees

• Current Assignee: Cerus Corporation
• Original Assignee: David P. Hearst, George D. Cimino, John E. Hearst, Stephen T. Isaacs
• Inventors: Hearst, John E., Cimino, George D., Isaacs, Stephen T., Hearst, David P.
• Primary Examiner(s): Berman, Jack I.
• Assistant Examiner(s): NGUYEN, KIET TUAN
• Application Number:
  US07/428,510
• Time in Patent Office: 1,195 Days
• Field of Search: 250/455.1, 250/453.1, 250/454.1, 250/504 R, 422/186, 422/186.01, 422/186.3
• US Class Current: 250/455.11
• CPC Class Codes: B01J 19/123 Ultraviolet light

Abstract

A device comprising a light source, a sample holder and a temperature control chamber. The sample holder supports the vessels in a fixed relationship relative to the light source. The temperature control chamber surrounds the sample holder and vessels, so that a temperature control fluid may be passed around the sample vessels effectively maintaining the temperature of the sample vessels within a desired temperature range.

Claims

1. A photoactivation device for treating photoreactive compounds, comprising:

a) means for continuously flowing sample liquid containing photoreactive compound;

b) means for providing appropriate wavelengths of electromagnetic radiation in a fixed relationship with said continuous flowing means for cause activation of at least one photoreactive compound; and

c) means for maintaining the temperature of said continuously flowing sample liquid within a desired temperature range during said activation.

2. A photoactivation device as in claim 1 further comprising means for containing said radiation providing means, such that a user is shielded from said wavelengths of electromagnetic radiation.

3. A photoactivation device as in claim 2 wherein said radiation containing means comprises an opaque housing surrounding said radiation providing means.

4. A photoactivation device as in claim 3 wherein said continuous flowing means comprises a chamber interior to said housing and positioned in a fixed relationship to said radiation providing means.

5. A photoactivation device as in claim 4 wherein said chamber has inlet and outlet ports so that sample liquid may enter and exit.

6. A device, comprising:

a) means for providing electromagnetic radiation;

b) means for supporting a plurality of sample vessels in a fixed relationship with said radiation providing means during irradiation;

c) an opaque housing for containing said radiation providing means; and

d) means for maintaining the temperature of same sample vessels within a desired temperature range during said irradiation comprising a chamber positioned interior to said housing and in a fixed relationship to said radiation providing means.

7. A device as in claim 6, wherein said sample vessel supporting means comprises intrusions in said chamber.

8. A device as in claim 6 wherein said chamber has exterior and interior walls.

9. A device as in claim 8, wherein said interior walls of said chamber form a trough.

10. A device as in claim 9 wherein said sample vessel supporting means comprises a sample rack detachably coupled to said housing above said trough.

11. A device as in claim 10, further comprising a sample cover dimensioned to overlay said sample rack.

12. A device as in claims 6, 7, 8, 9, 10 or 11 wherein said chamber has inlet and outlet ports so that temperature control liquid may enter and exit.

13. A device, comprising:

a) means for providing electromagnetic radiation, having a wavelength cutoff at 300 nanometers;

b) means for supporting a plurality of sample vessels in a fixed relationship with said radiation providing means during irradiation; and

c) means for maintaining the temperature of same sample vessels within a desired temperature range during said irradiation, comprising a chamber positioned in a fixed relationship to said radiation providing means.

14. A device as in claim 13, wherein said sample vessel supporting means comprises intrusions in said chamber.

15. A device as in claim 13, wherein said chamber has exterior and interior walls.

16. A device as in claim 15, wherein said interior walls of said chamber form a trough.

17. A device as in claim 16, wherein said sample vessel supporting means comprises a sample rack detachably coupled to said housing above said trough.

18. A device as in claim 17, further comprising a sample cover dimensioned to overlay said sample rack.

19. A device as in claims 13, 14, 15, 16, 17 or 18, wherein said chamber has inlet and outlet ports so that temperature control liquid may enter and exit.

20. A device, comprising:

a) a fluorescent source of ultraviolet radiation;

b) means for supporting a plurality of sample vessels in a fixed relationship with said fluorescent radiation source during irradiation; and

c) means for maintaining the temperature of said sample vessels within a desired temperature range during said irradiation, comprising a chamber positioned in fixed relationship to said fluorescent radiation source.

21. A device as in claim 20, wherein said sample vessel supporting means comprises intrusions in said chamber.

22. A device as in claim 20, wherein said chamber has exterior and interior walls.

23. A device as in claim 22, wherein said interior walls of said chamber form a trough.

24. A device as in claim 23, wherein said sample vessel supporting means comprises a sampler rack detachably coupled to said housing above said trough.

25. A device as in claim 24, further comprising a sample cover dimensioned to overlay said sample rack.

26. A device as in claims 20, 21, 22, 23, 24 or 25, wherein said chamber has inlet and outlet ports so that temperature control liquid may enter and exit.

27. A device, comprising:

a) a fluorescent source of ultraviolet radiation;

b) means for supporting a plurality of sample vessels, said sample supporting means positioned with respect to said fluorescent source, so that, when measured for the wavelengths between 200 to 400 nanometers, an intensity flux greater than 15 mW cm^-2 is provided to said sample vessels during irradiation with said fluorescent source;

c) an opaque housing for containing said radiation providing means; and

d) means for maintaining the temperature of said sample vessels within a desired temperature range during said activation, comprising a chamber positioned interior to said housing and in a fixed relationship to said fluorescent radiation source.

28. A device as in claim 27, wherein said sample vessel supporting means comprises intrusions in said chamber.

29. A device as in claim 27, wherein said chamber has exterior and interior walls.

30. A device as in claim 29, wherein said interior walls of said chamber form a trough.

31. A device as in claim 30, wherein said sample vessel supporting means comprises a sample rack detachably coupled to said housing above said trough.

32. A device as in claim 31, further comprising a sample overlay dimensioned to extend over and cover said sample rack.

33. A device as in claims 27, 28, 29, 30, 31 or 32, wherein said chamber has inlet and outlet ports so that temperature control liquid may enter and exit.