Immunological determination of free human protein S and C4bp-protein S complex
First Claim
1. A method of immunologically determining free human protein S in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, wherein each antibody binds to a distinct epitope of free human protein S, and either the primary or the secondary antibody, but not both, is a monoclonal antibody having a property of binding to free human protein S, and not binding to a complex of the human protein S and human complement cofactor C4b-binding protein (C4bp), said monoclonal antibody being produced by hybridoma FERM BP-2629 deposited in Fermentation Research Institute, Agency of Industrial Science and Technology, Japan and determinating the presence of said antibody.
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Abstract
A method of immunologically determining free human protein S in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, the primary and secondary antibodies having the property of binding to different epitopes of free human protein S, and one of the primary and secondary antibodies being a monoclonal antibody having the property of not binding to a complex of the human protein S and human complement cofactor C4b-binding protein (C4bp) but specifically binding to the free human protein S. Also provided is a method of immunologically determining a complex of human protein S and human complement cofactor C4b-binding protein (C4bp) in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, one of the primary and secondary antibodies being a monoclonal antibody having the property of not binding to free human protein S and human complement cofactor C4b-binding protein (C4bp) but binding specifically to the complex, and the other being an antibody having the property of binding to the human complement cofactor C4b-binding protein (4Cbp).
12 Citations
14 Claims
- 1. A method of immunologically determining free human protein S in an assay sample, which comprises contacting a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody with the assay sample, wherein each antibody binds to a distinct epitope of free human protein S, and either the primary or the secondary antibody, but not both, is a monoclonal antibody having a property of binding to free human protein S, and not binding to a complex of the human protein S and human complement cofactor C4b-binding protein (C4bp), said monoclonal antibody being produced by hybridoma FERM BP-2629 deposited in Fermentation Research Institute, Agency of Industrial Science and Technology, Japan and determinating the presence of said antibody.
- 2. A monoclonal antibody produced by hybridoma FERM BP-2629 deposited in Fermentation Research Institute, Agency of Industrial Science and Technology, Japan.
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3. A hybridoma FERM BP-2629 deposited in Fermentation Research Institute, Agency of Industrial Science and Technology, Japan.
- 4. A reagent system for immunological determination of free human protein S in an assay sample, comprising a primary antibody fixed to an insoluble solid carrier and a labelled secondary antibody, wherein each antibody binds to a distinct epitope of free human protein S, and either the primary or the secondary antibody, but not both, is a monoclonal antibody having a property of binding to free human protein S and not binding to a complex of the human protein S and human complement cofactor C4b-binding protein (C4bp), said monoclonal antibody being produced by hybridoma FERM BP-2629 deposited in Fermentation Research Institute, Agency of Industrial Science and Technology, Japan.
Specification