Leukaemia inhibitory factor

• US 5,187,077 A
• Filed: 12/09/1988
• Issued: 02/16/1993
• Est. Priority Date: 04/02/1987
• Status: Expired due to Term

• Current Assignee: Chemicon International, Inc. (Merck & Co., Inc.)
• Original Assignee: Zenyth Therapeutics Limited (CSL Limited)
• Inventors: Gearing, David P., Nicola, Nicos A., Metcalf, Donald, Willson, Tracy A., Hilton, Douglas J., Simpson, Richard J., Gough, Nicholas M., King, Julie A., Nice, Edouard C.
• Primary Examiner(s): Ivy, C. Warren
• Assistant Examiner(s): Owens, A. A.
• Application Number:
  US07/294,514
• Time in Patent Office: 1,530 Days
• Field of Search: 536/27, 435/69.1, 435/69.5, 435/320.1, 435/255, 435/252.3, 435/252.33, 435/240.2
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 35/02 specific for leukemia
• CPC Class Codes: C07K 14/5415 Leukaemia inhibitory factor [LIF]
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/02 containing a signal sequence
• CPC Class Codes: C07K 2319/23 containing a GST-tag
• CPC Class Codes: C07K 2319/50 containing protease site
• CPC Class Codes: C07K 2319/74 containing a fusion for binding to a cell surface receptor
• CPC Class Codes: C07K 2319/95 containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)
• CPC Class Codes: C12N 15/81 for yeasts
• CPC Class Codes: C12N 15/86 Viral vectors
• CPC Class Codes: C12N 2740/13043 viral genome or elements thereof as genetic vector
• CPC Class Codes: C12P 21/02 having a known sequence of two or more amino acids, e.g. glutathione
• CPC Class Codes: C12P 21/06 produced by the hydrolysis of a peptide bond, e.g. hydrolysate products preparing foodstuffs by protein hydrolysis A23J3/00

Abstract

A leukaemia-inhibitory factor (LIF) is disclosed, together with a method of preparation of LIF in essentially pure form. Nucleotide and amino acid sequences are disclosed, together with recombinant DNA moleclues and host cells for production of polypeptides having LIF activity.

Claims

1. A recombinant DNA molecule comprising a nucleotide sequence which codes for a polypeptide having LIF activity.

3. The molecule of claim 1, wherein said nucleotide sequence hybridizes to a nucleic acid probe corresponding to a subsequence of a LIF encoding insert selected from the group consisting of the LIF encoding inserts of clones pLIF7.2b, pLIFNK2, pLIFNK3, and pHGLIFBam1.

4. The molecule of claim 1, further comprising a promoter sequence operably linked to said nucleotide sequence is rendered said molecule being suitable for use as an expression vector.

5. A host cell transformed with a recombinant DNA molecule according to claim 4, wherein said cell expresses, under the control of said promoter sequence, a polypeptide having LIF activity.

6. The recombinant DNA molecule of claim 1 in which the polypeptide is human LIF.

7. The recombinant DNA molecule of claim 1 in which the polypeptide is murine LIF.

2. A recombinant DNA molecule comprising a nucleotide sequence which codes for a polypeptide having the amino acid sequence selected from the group consisting of those set forth in FIGS. 15, 26 and 29.