Apparatus and process for effecting sequential peptide syntheses

• US 5,188,733 A
• Filed: 02/26/1992
• Issued: 02/23/1993
• Est. Priority Date: 02/26/1992
• Status: Expired due to Fees

• Current Assignee: Trustees of Boston University
• Original Assignee: Trustees of Boston University
• Inventors: Wang, Zhengxin, Laursen, Richard A.
• Primary Examiner(s): SPEAR JR, FRANK
• Application Number:
  US07/841,943
• Time in Patent Office: 363 Days
• Field of Search: 422/101, 422/102, 210/450, 210/323.1, 210/321.75, 210/321.84, 210/638
• US Class Current: 210/321.84
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00315 Microtiter plates
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: C07K 1/045 using devices to improve synthesis, e.g. reactors, special vessels
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries

Abstract

A chemically modified membrane is positioned between a first template having a first set of holes and a second set of holes aligned with the first set of holes to define a plurality of exposed portions of the membrane in such as manner as to prevent collapse of pores in the membrane during application of a differential pressure across the membrane.

Claims

1. Apparatus for effecting a plurality of independent chemical syntheses which comprises:

a first template having a first set of through-holes positioned in a pattern,

a second template having a second set of through-holes positioned in and aligned with said pattern,

a chemically modified porous polymeric membrane positioned between said first and said second template,

sealing means positioned between said first set of through-holes and second set of through-holes,

means for applying differential pressure to effect fluid flow from said first set of holes to said second set of holes

and means for preventing collapse of said membrane during application of said differential pressure,

said chemically modified membrane having a first chemical moiety which permits covalent binding thereof with a second chemical moiety having a reactive carboxyl group.

2. The apparatus of claim 1 wherein said first set of through-holes and said second set of through-holes comprises a plurality of holes.

3. The apparatus of claim 1 wherein said chemical moiety includes a labile linker.

4. The apparatus of claim 2 wherein said chemical moiety includes an acid labile linker.

5. The apparatus of claim 1 which includes means for applying a differential pressure across said membrane.

6. The apparatus of claim 2 which includes means for applying a differential pressure across said membrane.

7. The apparatus of claim 3 which includes means for applying a differential pressure across said membrane.

8. The apparatus of claim 4 which includes means for applying a differential pressure across said membrane.