4,7-dichlorofluorescein dyes as molecular probes

• US 5,188,934 A
• Filed: 11/14/1989
• Issued: 02/23/1993
• Est. Priority Date: 11/14/1989
• Status: Expired due to Term

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Woo, Sam, Lee, Linda G., Menchen, Steven M., Connell, Charles R., Fung, Steven, Chakerian, Vergine, Hershey, N. Davis
• Primary Examiner(s): YARBROUGH, AMELIA
• Application Number:
  US07/436,455
• Time in Patent Office: 1,197 Days
• Field of Search: 435/6, 435/91, 435/172.3, 435/968, 436/800, 549/224, 549/382, 935/78, 935/77
• US Class Current: 435/6.12
• CPC Class Codes: C07D 311/82 Xanthenes
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C09B 11/08 Phthaleins; Phenolphthaleins; Fluorescein
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2535/101 Sanger sequencing method, i.e. oligonucleotide sequencing using primer elongation and dideoxynucleotides as chain terminators
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: Y10S 435/968 High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine

Abstract

Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-4,7-dichlorofluoresceins. Methods of using the dyes in automated DNA sequencing are described.

Claims

1. A compound having the formula:

##STR11## wherein;

A'"'"' is hydrogen, fluoro, chloro, or a group that may be converted to a linking functionality;

D'"'"' is fluoro, chloro, or an acidic anionic group;

X'"'"' is hydrogen, fluoro, or chloro;

Z₃ and Z₄ are separately hydrogen, halo, lower alkyl, lower alkyloxy, or a group that may be converted to a linking functionality; and

wherein at least one of A'"'"', Z₃, and Z₄ is a group that may be converted to a linking functionality.

2. The compound of claim 1 wherein A'"'"' is carboxyl, sulfonyl, or amino;

B'"'"' is carboxyl or sulfonyl;

X'"'"' is hydrogen;

Z₃ and Z₄ are separately hydrogen, halo, methyl, methoxy, ethyl, ethoxy, carboxyl, sulfonyl, or methylamino.

3. The compound of claim 2 wherein only one of A'"'"', Z₃ and Z₄ is carboxyl, sulfonyl, methylamino, or amino.

4. The compound of claim 3 wherein A'"'"' and D'"'"' are carboxyl, Z₃ is hydrogen or chloro, and Z₄ is hydrogen or chloro.

5. A method of distinguishing polynucleotides having different terminal dideoxynucleotides in the chain termination method of DNA sequencing, the method comprising the steps of:

forming a mixture of a first, a second, a third, and a fourth class of polynucleotides,

each polynucleotide in the first class having a 3'"'"'-terminal dideoxyadenosine and being labeled with a first dye selected from the group consisting of 5- and 6-carboxyfluorescein, 5- and 6-carboxy-4,7-dichlorofluorescein, 2'"'"',7'"'"'-dimethoxy-5- and 6-carboxy-4,7-dichlorofluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-5- and 6-carboxyfluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein, 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-5- and 6-carboxy-4,7-dichlorofluorescein, and 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-4'"'"',5'"'"'-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein,

each polynucleotide in the second class having a 3'"'"'-terminal dideoxythymidine and being labeled with a second dye selected from the group consisting of 5- and 6-carboxyfluorescein, 5- and 6-carboxy-4,7-dichlorofluorescein, 2'"'"',7'"'"'-dimethoxy-5- and 6-carboxy-4,7-dichlorofluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-5- and 6-carboxyfluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein, 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-5- and 6-carboxy-4,7-dichlorofluorescein, and 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-4'"'"',5'"'"'-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein,

each polynucleotide in the third class having a 3'"'"'-terminal dideoxyguanosine and being labeled with a third dye selected from the group consisting of 5- and 6-carboxyfluorescein, 5- and 6-carboxy-4,7-dichlorofluorescein, 2'"'"',7'"'"'-dimethoxy-5- and 6-carboxy-4,7-dichlorofluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-5- and 6-carboxyfluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein, 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-5- and 6-carboxy-4,7-dichlorofluorescein, and 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-4'"'"',5'"'"'-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein,

each polynucleotide in the fourth class having a b 3'"'"'-terminal dideoxycytosine and being labeled with a fourth dye selected from the group consisting of 5- and 6-carboxyfluorescein, 5- and 6-carboxy-4,7-dichlorofluorescein, 2'"'"',7'"'"'-dimethoxy-5- and 6-carboxy-4,7-dichlorofluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-5- and 6-carboxyfluorescein, 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein, 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-5- and 6-carboxy-4,7-dichlorofluorescein, and 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-4'"'"',5'"'"'-dichloro-5- and 6-carboxy-4,7-dichlorofluorescein,

wherein the first, second, third, and fourth dyes are spectrally resolvable from one another;

electrophoretically separating on a gel the polynucleotides in the mixture so that bands of similarly sized polynucleotides are formed;

illuminating with an illumination beam the bands on the gel, the illumination beam being capable of causing the dyes to fluoresce; and

identifying the class of the polynucleotides in the bands by the fluorescence or absorption spectrum of the dyes.

6. The method of claim 5 wherein each polynucleotide of said first class is labeled by attaching said first dye to said 3'"'"'-terminal dideoxyadenosine by way of a linking group, each polynucleotide of said second class is labeled by attaching said second dye to said 3'"'"'-terminal dideoxythymidine by way of a linking group, each polynucleotide of said third class is labeled by attaching said third dye to said 3'"'"'-terminal dideoxyguanosine by way of a linking group, and each polynucleotide of said fourth class is labeled by attaching said fourth dye to said 3'"'"'-terminal dideoxycytosine by way of a linking group.

7. The method of claim 6 wherein said dideoxyadenosine is 2'"'"',3'"'"'-dideoxy-7-deazaadenosine, said dideoxycytidine is 2'"'"',3'"'"'-dideoxycytidine, said dideoxyguanosine is 2'"'"',3'"'"'-dideoxy-7-deazaguanosine or 2'"'"',3'"'"'-dideoxy-7-deazainosine, and said dideoxythymidine is 2'"'"',3'"'"'-dideoxyuridine.

8. The method of claim 7 wherein said linking group links a 5 carbon of said 2'"'"',3'"'"'-dideoxycytidine or 2'"'"',3'"'"'-dideoxyuridine to a 5 or 6 carbon of said second dye or said fourth dye, respectively, and wherein said linking group links a 7 carbon of said 2'"'"',3'"'"'-dideoxy-7-deazaadenosine or 2'"'"',3'"'"'-dideoxy-7-guanosine or 2'"'"',3'"'"'-dideoxy-7-deazainosine to a 5 or 6 carbon of said first dye or said third dye, respectively.

9. The method of claim 8 wherein said linking group is carboxyaminoalkynyl.

10. The method of claim 9 wherein said carboxyaminoalkynyl is 3-carboxyamino-1-propynyl.

11. The method of claim 5 wherein said dideoxyadenosine is 2'"'"',3'"'"'-dideoxy-7-deazaadenosine, said dideoxycytidine is 2'"'"',3'"'"'-dideoxycytidine, said dideoxyguanosine is 2'"'"',3'"'"'-dideoxy-7-deazaguanosine or 2'"'"',3'"'"'-dideoxy-7-deazainosine, and said dideoxythymidine is 2'"'"',3'"'"'-dideoxyuridine.

12. The method of claim 11 wherein said first dye is 2'"'"',7'"'"'-dimethoxy-5-carboxy-4,7-dichlorofluorescein, said fourth dye is 5-carboxy-4,7-dichlorofluorescein, said third dye is 2'"'"',7'"'"'-dimethoxy-4'"'"',5'"'"'-dichloro-6-carboxy-4,7-dichlorofluorescein or 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-4'"'"',5'"'"'-dichloro-5-carboxy-4,7-dichlorofluorescein, and said second dye is 1'"'"',2'"'"',7'"'"',8'"'"'-dibenzo-5-carboxy-4,7-dichlorofluorescein.

13. In a chain termination method of DNA sequencing, the method of the type wherein four classes of DNA fragments are formed such that DNA fragments of the same class have the same terminating base and are labelled with the same fluorescent dye, an improvement comprising:

labelling DNA fragments of at least one class with a 4,7-dichlorofluorescein dye selected from the group defined by the formula;

##STR12## wherein;

A'"'"' is hydrogen, fluoro, chloro, or a group that may be converted to a linking functionality;

D'"'"' is fluoro, chloro, or an acidic anionic group;

X'"'"' is hydrogen, fluoro, or chloro;

Z'"'"' is hydrogen or, when taken with Z₂, benzo;

Z₂, when taken along, is hydrogen, halo, lower alkyl, lower alkyloxy, or a group that may be converted to a linking functionality, or when taken with Z₁, Z₂ is benzo;

Z₃ and Z₄ are separately hydrogen, halo, lower alkyl, lower alkyloxy, or a group that may be converted to a linking functionality;

Z₆ is hydrogen or, when taken with Z₅, benzo;

Z₅, when taken alone, is hydrogen, halo, lower alkyl, lower alkyloxy, or a group that may be converted to a linking functionality, or when taken with Z₆, benzo; and

wherein only one of A'"'"', A₂, Z₃, Z₄, and Z₅ is a group that may be converted to a linking functionality.

14. The method of claim 13 wherein A'"'"' and D'"'"' are carboxyl;

X'"'"' is hydrogen;

Z₂, when taken alone, is hydrogen, chloro, methyl, ethyl, ethoxy, or methoxy;

Z₃ and Z₄ are separately hydrogen, chloro, methoyl, ethyl, methoxy, or ethyoxy; and

Z₅, when taken alone, is hydrogen, chloro, methyl, ethyl, ethoxy, or methoxy.

15. The method of claim 14 wherein Z₁, Z₃, Z₄, and Z₆ are hydrogen, and Z₂ and Z₅ are methoxy.

16. The method of claim 14 wherein Z₁ and Z₆ are hydrogen, Z₃ and Z₄ are chloro, and Z₂ and Z₅ are methoxy, or wherein Z₁ and Z₂ are benzo, Z₅ and Z₆ are benzo, and Z₃ and Z₄ are chloro.

17. The method of claim 14 wherein Z₁ and Z₂ taken together are benzo, Z₅ and Z₆ taken together are benzo, and Z₃ and Z₄ are hydrogen.

18. The method of claim 14 wherein R₁, R₂, R₃, R₄, R₅, and R₆ are hydrogen.