Device for processing biological specimens for analysis of nucleic acids

• US 5,188,963 A
• Filed: 11/17/1989
• Issued: 02/23/1993
• Est. Priority Date: 11/17/1989
• Status: Expired due to Fees

• Current Assignee: Gene Tec Corporation
• Original Assignee: Gene Tec Corporation
• Inventors: Stapleton, Marilyn J.
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/438,592
• Time in Patent Office: 1,194 Days
• Field of Search: 422/56.58, 422/69, 422/101, 422/102, 422/104, 436/177, 436/178, 436/515, 436/516, 435/287, 435/299, 204/182.6, 204/182.8, 204/182.9, 204/299 R
• US Class Current: 435/288.3
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/043 Hinged closures
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: C12N 15/00 Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor mutants or genetically engineered microorganisms, per se C12N1/00, C12N5/00, C12N7/00; new plants per se A01H; plant reproduction by tissue culture techniques A01H4/00; new animals per se A01K67/00; use of medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases, gene therapy A61K48/00
• CPC Class Codes: C12Q 1/6841 In situ hybridisation
• CPC Class Codes: C12Q 2543/101 in situ amplification
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels
• CPC Class Codes: G01N 1/31 Apparatus therefor
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

The matrix carrier is a hinged compartment facilitating automation of DNA- and RNA-based diagnostics and genetic surveillance and detection. Specimens are embedded in a matrix in the carrier. The matrix is then treated by one or more of the techniques such as amplification, electrophoresis, and hybridization as selected for the desired analysis and then the sample is treated to detect the cellular component.

Claims

1. A carrier device for specimen handling for analysis of a specimen for cellular components, comprising:

a top cover portion and a bottom housing portion, said top cover portion movable between an open and a closed position;

said top cover portion having a first area that extends beyond a first side of said bottom housing portion;

said top cover portion and said bottom housing portion connected together by way of a hinge along one side of said bottom housing portion, wherein said top cover portion hingedly moves away from the bottom housing portion and upward from the closed position to an open position; and

said bottom housing portion having edges on second and third sides and an extended area on a fourth side of said bottom housing portion extending beyond said top cover portion, said side edges defining a space called the matrix and specimen holding area;

opposing surfaces of said top and bottom portions defining a top and bottom of said matrix and specimen holding area when said top cover portion is in a closed position;

said space being in fluid communication with and extending to the extended area;

said extended area containing a fluid receiving area; and

wherein when said top cover portion is closed and aligned with respect to the side edges of the bottom housing portion, the top cover portion covers the matrix and specimen holding area, and fluids added to said fluid receiving area fill said matrix and specimen holding area;

wherein excess fluids exit the matrix and specimen holding area at an end of the matrix and specimen holding area opposite the fluid receiving area.

2. A carrier device according to claim 1, wherein the top cover portion is hinged to the bottom housing portion along the first side of said bottom housing portion.

3. A carrier device according to claim 1, wherein the top cover portion is hinged to the bottom housing portion along second side of said bottom housing portion, and said second side is perpendicular to said first side of said bottom housing portion.

4. A carrier device according to claim 1, wherein the matrix and specimen holding area is divided into sections such that certain of the sections may be selected to individually treat specimens.

5. A carrier device according to claim 1, further comprising a matrix material containing a specimen in said matrix and specimen holding area;

wherein fluids added to the fluid receiving area after the matrix material and specimen are in the matrix and specimen holding area form a liquid film in contact with the top cover portion and the matrix and specimen material when said top cover potion is in the closed position.

6. A carrier device according to claim 5, wherein said matrix material is selected from a group consisting of agarose and polyacrylamide.

7. A carrier device according to claim 6, wherein openings are positioned towards opposite ends of the carrier to allow wires to make electrical contact directly with the matrix material in the matrix and specimen holding area and indirectly through fluids in the fluid receiving area;

wherein the carrier is structured so a current may be applied that permits electrophoretic transfer of macromolecules from one matrix section to another.

8. A carrier according to claim 5, wherein the carrier is made of material sufficient to withstand being heated and cooled rapidly by direct contact with a platform heater between temperatures required for melting agarose, rendering specimen non-infectious and denaturing DNA and temperatures required for complexation reactions within the specimen.

9. A carrier device according to claim 1, wherein the structure of the carrier allows fluids to behave so that the first fluid applied to fluid receiving area spreads into a liquid film over the matrix and specimen holding area;

wherein a second fluid added to said fluid receiving area is capable of replacing first fluid in said fluid receiving area and the liquid film over the matrix and specimen holding area;

wherein fluids added one after another flow through the carrier in one direction.

10. A carrier device according to claim 1, wherein at least one piece of said top and bottom portions is constructed of multiple pieces and at least one of the pieces is made of an optically clear material.

11. A carrier device according to claim 1, wherein each of a plurality of said carrier devices has structures for placing them together within a processor-controlled apparatus.

12. A carrier device for specimen handling for analysis of a specimen for cellular components, comprising:

a top cover portion and a bottom housing portion movable between an open position and a closed position, said bottom housing portion having an extended area on a first side of said bottom housing portion extending beyond said top cover portion;

wherein said bottom housing portion has edges defining a space, said space comprises a fluid receiving area in the extended area and a matrix and specimen holding area;

said fluid receiving area being in fluid communication with said matrix and specimen holding area;

wherein a matrix and a specimen may be added to the matrix and specimen holding area;

wherein the top cover portion covers said matrix and specimen holding area when said top cover portion is in the closed position; and

wherein fluids added to said fluid receiving area fill said matrix and specimen holding area forming a liquid film in contact with the top cover portion and the matrix and specimen;

wherein excess fluids exit the matrix and specimen holding area opposite where the fluids are added.