Reagent source for chemiluminescent reactions, test kit, and method for use

• US 5,188,965 A
• Filed: 04/09/1992
• Issued: 02/23/1993
• Est. Priority Date: 03/18/1991
• Status: Expired due to Term

• Current Assignee: Difco Laboratories, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Difco Laboratories, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Wannlund, Jon C.
• Primary Examiner(s): Rollins, John W.
• Application Number:
  US07/867,280
• Time in Patent Office: 320 Days
• Field of Search: 436/165, 436/166
• US Class Current: 436/165
• CPC Class Codes: C12Q 1/66 involving luciferase
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

At least some of the reagents required for a chemiluminescent reaction are dried on a piece of a carrier material. The reagents are stable for extended periods of time, without refrigeration, in this form. The sheet of carrier material is of a shape and size to fit flat against the interior surface of a transparent wall of a test well through which light output of the reaction is measured. Light output is thereby concentrated in a region adjacent the wall, minimizing attenuation of the light in passing from the well to permit measurement of low light output levels.

Claims

1. A reagent source for use in a test wherein chemiluminescent light output is measured from a test well having a transparent wall of a preselected peripheral shape and size through which the light output is measured, comprising:

a sheet of solid carrier material having a portion of the reactants required for a chemiluminescent chemical reaction contained therein in a solid form.

2. The reagent source of claim 1, wherein the sheet has a size and shape such that it fits flat against an interior surface of a transparent wall of a test well having a preselected peripheral shape and size through which the light output is measured.

3. The reagent source of claim 1, wherein the carrier material is paper.

4. The reagent source of claim 1, wherein the reactant contained within the carrier material includes luciferase.

5. The reagent source of claim 1, wherein the reactant contained within the carrier material includes luciferin.

6. The reagent source of claim 1, wherein the sheet of solid carrier material is round with a diameter of about 5 millimeters.

7. The reagent source of claim 1, further including

a second sheet of solid carrier material having at least one of the reactants required for a chemiluminescent chemical reaction contained therein in a solid form.

8. A test kit for use in measurements of chemiluminescent light output, comprising:

a test well having a transparent wall with an interior surface; and

a sheet of solid carrier material having a portion of the reactants required for a chemiluminescent chemical reaction contained therein in a solid form, the sheet having a size and shape such that it fits against the interior surface of the transparent wall of the test well.

9. The test kit of claim 8, wherein the test well is supported in a test plate.

10. The test kit of claim 8, wherein the carrier material is paper.

11. The test kit of claim 8, wherein the reactant contained within the carrier material includes luciferase.

12. The test kit of claim 8, wherein the reactant contained within the carrier material includes luciferin.

13. The test kit of claim 8, further including

means for restraining the sheet of solid carrier material from movement away from the interior surface of the transparent wall of the test well.

14. The test kit of claim 13, wherein the means for restraining includes

a cover sheet fixed to the surface of the test plate, and

a length of a restraining member captured between the cover sheet and the sheet of carrier material.

15. The test kit of claim 9, further including a plurality of test wells supported in the test plate.

16. A process for performing a test for the presence of a reactant in a sample fluid, comprising the steps of:

furnishing a test well having a transparent wall of a preselected peripheral shape and size;

furnishing a sheet of solid carrier material having a portion of the reactants required for a chemiluminescent chemical reaction contained therein in a solid form, the sheet having a size and shape such that it fits flat against an interior surface of the transparent wall of the test well;

adding to the test well a sample fluid that may contain a portion of the reactants required for the chemiluminescent chemical reaction, the test well and the sample fluid together containing all of the reactants required for the chemiluminescent chemical reaction in the event of a positively testing sample but not containing all of the reactants required for the chemiluminescent chemical reaction in the event of a negatively testing sample; and

measuring the light output of the chemiluminescent chemical reaction, if any, through the transparent wall of the test well.

17. The process of claim 16, wherein the step of furnishing a sheet of solid carrier material includes the steps of:

preparing a liquid solution of the portion of the reactants in a liquid carrier,

applying a quantity of the liquid solution to a piece of untreated carrier material to permit the liquid solution to soak into the carrier material, and

evaporating the liquid carrier to leave the portion of reactants in the carrier material.

18. The process of claim 16, wherein the sample fluid is at least partially opaque to visible light.

19. The process of claim 16, wherein the carrier material is paper.