Multi-colored electrophoresis pattern reading system

• US 5,190,632 A
• Filed: 03/20/1992
• Issued: 03/02/1993
• Est. Priority Date: 03/22/1991
• Status: Expired due to Term

• Current Assignee: Hitachi Software Engineering Co., Ltd. (Hitachi, Ltd.)
• Original Assignee: Hitachi Software Engineering Co., Ltd. (Hitachi, Ltd.)
• Inventors: Nasu, Hisanori, Fujimiya, Hitoshi
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US07/854,872
• Time in Patent Office: 347 Days
• Field of Search: 204/299 R, 204/182.8, 356/344
• US Class Current: 204/608
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

The multi-colored electrophoresis pattern reading system compares the patterns of electrophoresis without a warp to be caused upon electrophoresis by reading the patterns of electrophoresis which is carried out concurrently for plural samples and by labelling the samples with fluorescent substances having different fluorescent wavelengths. The patterns of electrophoresis are read by allowing fluorescence to emit from the plural samples. In reading the fluorescent patterns, optical signals are received by the light receiving section and then the fluorescence having a predetermined wavelength is separated by the fluorescent-wavelength separating section from the optical signals with the aid of an optical filter capable of controlling an angle of incidence relative to the optical axis of the optical filter. The optical signals passed through the fluorescent-wavelength separating section is then subjected to optoelectrical conversion into electric signals which in turn are subjected to signal processing, thereby converting the electric signals into a desired form of data representation.

Claims

1. A multi-colored electrophoresis pattern reading system capable of labelling each of plural samples separately with each of plural fluorescent substances having a different fluorescence wavelength, subjecting the plural samples to electrophoresis to develop a pattern of electrophoresis, exciting the fluorescent substances labelled on the respective plural samples to emit fluorescence, and reading a fluorescent pattern emitting the fluorescence, comprising:

a light source means for irradiating the pattern of electrophoresis with irradiating light for exciting the fluorescent substance labelled on the sample to produce fluorescence;

a light scanning means for scanning the irradiating light while irradiating a gel in the direction of thickness of the gel with the irradiating light from the light source means;

a light receiving means for receiving the fluorescence separated from scattered light resulting form a reading surface on the basis of a position relationship of a light receiving path by setting a light receiving surface so as to be located in a direction different from an optical axis of the irradiating light;

a fluorescent-wavelength separating means for separating fluorescence having a predetermined wavelength from an optical signal received by the light receiving means, and having a separator and means capable of controlling an angle of incidence relative to an optical axis of the separator;

an optoelectric conversion means for generating electric signals by subjecting the optic signals passed through the fluorescent-wavelength separating means to optoelectrical conversion; and

a signal processing means for subjecting the electric signals generated by the optoelectric conversion means to signal processing and converting the electric signals into a predetermined form of data representation.

2. A multi-colored electrophoresis pattern reading system as claimed in claim 1, wherein said signal processing means has:

an optical filter as the separator;

an integral circuit composed of a condenser and a switch for controlling an integral operation;

an integral circuit for controlling the integral operation in synchronization with a scan of irradiating light by said light scanning means; and

means for changing an integral time and the number of scans for reading in accordance with the angle of incidence of the optical signals in the optical filter of said fluorescent-wavelength separating means.

3. A multi-colored electrophoresis pattern reading system as claimed in claim 1, wherein the fluorescent-wavelength separating means has a diffraction grating as the separator.

4. A multi-colored electrophoresis pattern reading system as claimed in claim 3, wherein said signal processing means has:

an integral circuit composed of a condenser and a switch for controlling an integral operation;

an integral circuit for controlling the integral operation in synchronization with a scan of irradiating light by said light scanning means; and

means for changing an integral time and the number of scans for reading in accordance with the angle of incidence of the optical signals in the differential grating of said fluorescent-wavelength separating means.

5. A multi-colored electrophoresis pattern reading system as claimed in claim 1, wherein said signal processing means further comprises an image compressing means for compressing an image by giving a differential value obtainable by subtracting a value of a pixel positioned at a right side of each line from a value of a pixel positioned at the left side thereof, wherein image data read from the pattern of electrophoresis is stored as a compressed differential value.

6. A system of a multi-colored fluorescent type for determining a sequence of bases of a nucleic acid with a multi-colored electrophoresis pattern reading system as claimed in claim 1, further comprising:

data processing means for deciding the sequence of the bases of the nucleic acid from image data of plural patterns of electrophoresis obtainable by the signal processing means; and

wherein electrophoresis of plural samples is carried out and the sequence of the bases of the nucleic acid is determined from the plural patterns of electrophoresis.

7. A system of a multi-colored fluorescent type for determining a sequence of bases of a nucleic acid wherein a plurality of multi-colored electrophoresis pattern reading systems as claimed in claim 1 are disposed, each reading system comprising:

a light source means for irradiating the pattern of electrophoresis with irradiating light for exciting the fluorescent substance labelled on the sample to produce fluorescence;

a light scanning means for scanning the irradiating light and irradiating a gel in the direction of thickness of the gel with the irradiating light from the light source means;

a light receiving means for receiving the fluorescence separated from scattered light resulting from a reading surface on the basis of a position relationship of a light receiving path by setting a light receiving surface so as to be located in a direction different from an optical axis of the irradiating light;

a fluorescent-wavelength separating means for separating fluorescence having a predetermined wavelength from an optical signal received by the light receiving means with an optical filter capable of controlling an angle of incidence relative to an optical axis of the optical filter;

an optoelectric conversion means for generating electric signals by subjecting the optic signals passed through the fluorescent-wavelength separating means to optoelectrical conversion;

a signal processing means for subjecting the electric signals generated by the optoelectric conversion means to signal processing and converting the electric signals into a predetermined form of data representation;

a data processing means for determining the sequence of the bases of the nucleic acid from the plural patterns of electrophoresis obtained by said signal processing means of each of the reading systems;

wherein the plurality of the multi-colored electrophoresis pattern reading systems are operated concurrently with each other; and

said data processing means of each of said reading systems reads the sequence of the bases of the nucleic acid from the plural patterns of electrophoresis obtained by said signal processing means of each of said reading systems.