Method of administering proteins to living skin cells

• US 5,190,762 A
• Filed: 01/23/1991
• Issued: 03/02/1993
• Est. Priority Date: 07/06/1988
• Status: Expired due to Term

• Current Assignee: Applied Kinetics Inc.
• Original Assignee: Applied Kinetics Inc.
• Inventors: Yarosh, Daniel B.
• Primary Examiner(s): Patterson, Jr., Charles L.
• Application Number:
  US07/644,920
• Time in Patent Office: 769 Days
• Field of Search: 424/450, 424/94.5, 435/193
• US Class Current: 424/450
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 9/127 Liposomes
• CPC Class Codes: C12N 9/1007 Methyltransferases (general) (2.1.1.)
• CPC Class Codes: C12N 9/22 Ribonucleases RNAses, DNAses catalytic nucleic acids C12N15/113

Abstract

A method for administering a protein having intracellular biological activity into the interior of living skin cells, which lie below the skin'"'"'s stratum corneum, is provided. The method comprises the steps of: (a) encapsulating the protein in liposomes; and (b) applying the liposomes to the outer surface of living skin so that the protein encapsulated in the liposomes traverses the skin'"'"'s stratum corneum and the outer membranes of said cells and is thereby delivered by the liposomes into the interior of said cells. In certain preferred embodiments, the liposomes are pH sensitive liposomes. In other preferred embodiments, the protein is a DNA repair enzyme, such as T4 endonuclease V.

Claims

1. A method for administering a protein having intracellular biological activity into the interior of living skin cells which lie below the skin'"'"'s stratum corneum, comprising the steps of:

(a) encapsulating the protein in liposomes; and

(b) applying the liposomes to the outer surface of living skin so that the protein encapsulated in the liposomes traverses the skin'"'"'s stratum corneum and the outer membranes of said cells and is thereby delivered by the liposomes into the interior of said cells.

2. The method of claim 1 wherein the liposomes are pH sensitive liposomes.

3. The method of claim 1 wherein the protein having intracellular biological activity is an enzyme.

4. The method of claim 3 wherein the enzyme is a DNA repair enzyme.

5. The method of claim 4 wherein the DNA repair enzyme is T4 endonuclease V.