Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA

• US 5,190,931 A
• Filed: 11/15/1989
• Issued: 03/02/1993
• Est. Priority Date: 10/20/1983
• Status: Expired due to Term

• Current Assignee: The Research Foundation for The State University of New York (State University of New York)
• Original Assignee: The Research Foundation for The State University of New York (State University of New York)
• Inventors: Inouye, Masayori
• Primary Examiner(s): Low, Christopher S. F.
• Application Number:
  US07/436,598
• Time in Patent Office: 1,203 Days
• Field of Search: 435/91, 435/172.3, 435/252.3, 435/252.33, 435/948, 435/235, 435/240.2, 536/27, 514/43, 514/44, 935/33, 935/34, 935/35, 935/36, 935/37, 424/93
• US Class Current: 435/91.32
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/111 spanning the whole gene, or a large part of it

Abstract

Gene expression in a cell can be regulated or inhibited by incorporating into or associating with the genetic material of the cell a non-native nucleic acid sequence which is transcribed to produce an mRNA which is complementary to and capable of binding to the mRNA produced by the genetic material of said cell.

Claims

1. A prokaryotic or eukaryotic cell containing a non-native DNA construct, which construct produces an RNA which regulates the function of a gene, said DNA construct containing the following operably linked DNA segments:

a. a transcriptional promoter segment;

b. a transcription termination segment; and

therebetween

c. a DNA segment;

whereby transcription of the DNA segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.

3. A method of regulating the function of a gene in a prokaryotic or eukaryotic cell which comprises introducing into said cell the DNA construct of claim 1.

4. A method of regulating the function of a gene in a prokaryotic or eukaryotic cell which comprises:

introducing into said cell the DNA construct of claim 1 whereby a transformed cell is obtained; and

growing said transformed cell whereby the RNA produced by transcription of said DNA segment regulates the functioning of said gene.

2. A prokaryotic or eukaryotic cell containing a non-native DNA construct, which construct produces an RNA which regulates the function of a gene, said DNA construct containing the following operably linked DNA segments:

a. a transcriptional promoter segment;

b. a transcription termination segment; and

c. a DNA segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.

5. A non-native DNA construct which, when present in a prokaryotic or eukaryotic cell containing a gene, produces an RNA which regulates the function of said gene, said DNA construct containing the following operably linked DNA segments:

a. a transcriptional promoter segment;

b. a transcription termination segment; and

c. a DNA segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.

7. A method of regulating the function of a gene in a prokaryotic or eukaryotic cell which comprises introducing into said cell the DNA construct of claim 5.

8. A method of regulating the function of a gene in a prokaryotic or eukaryotic cell which comprises:

introducing into said cell the DNA construct of claim 5 whereby a transformed cell is obtained; and

growing said transformed cell whereby the RNA produced by transcription of the inverted gene segment regulates the functioning of said gene.

6. A non-native DNA construct which, when present in a prokaryotic or eukaryotic cell containing a gene, produces an RNA which regulates the function of said gene, said DNA construct containing the following operably linked DNA segments:

a. a transcriptional promoter segment;

b. a transcription termination segment; and

therebetween

c. a DNA segment;

whereby transcription of the DNA segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.

9. A non-native polynucleotide construct which, when present in a cell containing a gene, produces an RNA which regulates the function of said gene, said polynucleotide construct containing the following operably linked polynucleotide segments:

a. a transcriptional promoter segment;

b. a transcription termination segment; and

c. a polynucleotide segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.

10. The polynucleotide construct of claim 9 wherein said cell is prokaryotic.

11. The polynucleotide construct of claim 9 wherein said cell is eukaryotic.

12. A polynucleotide construct of claim 9 wherein said transcriptional promoter segment comprises an inducible promoter.

13. A polynucleotide construct of claim 9 wherein said gene is an oncogene.

14. A polynucleotide construct of claim 9 wherein said gene is a viral gene.

15. A polynucleotide construct of claim 9 wherein said gene encodes a protein.

16. A polynucleotide construct of claim 15 wherein said transcriptional promoter segment comprises an inducible promoter.

17. A polynucleotide construct of claim 15 wherein said gene segment includes the 5'"'"' non-coding region of said gene.

18. A polynucleotide construct of claim 15 wherein said gene segment includes the ribosome binding portion of said gene.

19. A polynucleotide construct of claim 15 wherein said gene segment includes the translation initiation portion of said gene.

20. A vector having incorporated therein a polynucleotide construct according to any one of claims 9-19.

21. A vector according to claim 20 wherein said vector is a plasmid.

22. A vector according to claim 20 wherein said vector is a viral vector.

23. A pharmaceutical composition which comprises the polynucleotide construct of any one of claims 9-19.

24. A pharmaceutical composition which comprises the vector of claim 20.

25. A pharmaceutical composition which comprises the vector of claim 21.

26. A pharmaceutical composition which comprises the vector of claim 22.

27. A method of regulating the function of a gene in a cell which comprises introducing into said cell the polynucleotide construct of any one of claims 9 or 12-19.

28. A method of regulating the function of a gene in a cell which comprises introducing into said cell the vector of claim 20.

29. The method of claim 27 wherein said cell is prokaryotic.

30. The method of claim 27 wherein said cell is eukaryotic.

31. The method of claim 28 wherein said cell is prokaryotic.

32. The method of claim 28 wherein said cell is eukaryotic.

33. A method of regulating the function of a gene in a cell which comprises:

introducing into said cell the polynucleotide construct of any one of claims 9-19 whereby a transformed cell is obtained; and

growing said transformed cell whereby the RNA produced by transcription of the inverted gene segment regulates the functioning of said gene.

34. A non-native polynucleotide construct which, when present in a cell containing a gene, produces an RNA which regulates the function of said gene, said polynucleotide construct containing the following operably linked polynucleotide segments:

a. a transcriptional promoter segment;

b. a transcription termination segment; and

therebetween

c. a polynucleotide segment;

whereby transcription of the polynucleotide segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.

35. The polynucleotide construct of claim 34 wherein said cell is prokaryotic.

36. The polynucleotide construct of claim 34 wherein said cell is eukaryotic.

37. A polynucleotide construct of claim 34 wherein said transcriptional promoter segment comprises an inducible promoter.

38. A polynucleotide construct of claim 34 wherein said gene is an oncogene.

39. A polynucleotide construct of claim 34 wherein said gene is a viral gene.

40. A polynucleotide construct of claim 34 wherein said gene encodes a protein.

41. A polynucleotide construct of claim 40 wherein said transcriptional promoter segment comprises an inducible promoter.

42. A polynucleotide construct of claim 40 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to a 5'"'"' end non-coding portion of said ribonucleotide sequence transcribed from said gene.

43. A polynucleotide construct of claim 40 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to a ribosome binding portion of said ribonucleotide sequence transcribed from said gene.

44. A polynucleotide construct of claim 40 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to the translation initiation portion of said ribonucleotide sequence transcribed from said gene.

45. A vector having incorporated therein a polynucleotide construct according to any one of claims 34-44.

46. A vector according to claim 45 wherein said vector is a plasmid.

47. A vector according to claim 45 wherein said vector is a viral vector.

48. A pharmaceutical composition which comprises the polynucleotide construct of any one of claims 34-44.

49. A pharmaceutical composition which comprises the vector of claim 45.

50. A pharmaceutical composition which comprises the vector of claim 46.

51. A pharmaceutical composition which comprises the vector of claim 47.

66. A method of regulating the function of a gene in a cell which comprises introducing into said cell the polynucleotide construct of any one of claims 34 or 37-44.

67. A method of regulating the function of a gene in a cell which comprises introducing into said cell the vector of claim 45.

68. The method of claim 66 wherein said cell is prokaryotic.

69. The method of claim 66 wherein said cell is eukaryotic.

70. The method of claim 67 wherein said cell is prokaryotic.

71. The method of claim 67 wherein said cell is eukaryotic.

72. A method of regulating the function of a gene in a cell which comprises:

introducing into said cell the polynucleotide construct of any one of claims 34-44 whereby a transformed cell is obtained; and

growing said transformed cell whereby the RNA produced by transcription of said polynucleotide segment regulates the functioning of said gene.

52. A cell containing a non-native polynucleotide construct, which construct produces an RNA which regulates the function of a gene, said polynucleotide construct containing the following operably linked polynucleotide segments:

a. a transcriptional promoter segment;

b. a transcription termination segment; and

therebetween

c. a polynucleotide segment;

whereby transcription of the polynucleotide segment produces a ribonucleotide sequence which does not naturally occur in the cell, is complementary to a ribonucleotide sequence transcribed from said gene, and said non-naturally occurring ribonucleotide sequence regulates the function of said gene.

53. The cell of claim 52 wherein said cell is prokaryotic.

54. The cell of claim 52 wherein said cell is eukaryotic.

55. The cell of claim 52 wherein said transcriptional promoter segment comprises an inducible promoter.

56. The cell of claim 52 wherein said gene is an oncogene.

57. The cell of claim 52 wherein said gene is a viral gene.

58. The cell of claim 52 wherein said gene encodes a protein.

59. The cell of claim 58 wherein said transcriptional promoter segment comprises an inducible promoter.

61. The cell of claim 58 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to a ribosome binding portion of said ribonucleotide sequence transcribed from said gene.

62. The cell of claim 58 wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to the translation initiation portion of said ribonucleotide sequence transcribed from said gene.

63. The cell according to any one of claims 52-62 wherein said polynucleotide construct is incorporated into a vector.

64. The cell according to claim 63 wherein said vector is a plasmid.

65. The cell according to claim 63 wherein said vector is a viral vector.

60. The cell of claim wherein said polynucleotide segment encodes a ribonucleotide sequence complementary to a 5'"'"' end non-coding portion of said ribonucleotide sequence transcribed from said gene.

73. A cell containing a non-native polynucleotide construct, which construct produces an RNA which regulates the function of a gene, said polynucleotide construct containing the following operably linked polynucleotide segments:

a. a transcriptional promoter segment;

b. a transcription termination segment; and

c. a polynucleotide segment comprising a segment of said gene, said gene segment located between said promoter segment and said termination segment and being inverted with respect to said promoter segment and said termination segment, whereby the RNA produced by transcription of the inverted gene segment regulates the function of said gene.

74. The cell of claim 73 wherein said cell is prokaryotic.

75. The cell of claim 73 wherein said cell is eukaryotic.

76. The cell of claim 73 wherein said transcriptional promoter segment comprises an inducible promoter.

77. The cell of claim 73 wherein said gene is an oncogene.

78. The cell of claim 73 wherein said gene is a viral gene.

79. The cell of claim 73 wherein said gene encodes a protein.

80. The cell of claim 79 wherein said transcriptional promoter segment comprises an inducible promoter.

81. The cell of claim 79 wherein said gene segment includes the 5'"'"' non-coding region of said gene.

82. The cell of claim 79 wherein said gene segment includes the ribosome binding portion of said gene.

83. The cell of claim 79 wherein said gene segment includes the translation initiation portion of said gene.

84. The cell of any one of claims 79-83 wherein the polynucleotide construct is incorporated into a vector.

85. The cell of claim 84 wherein said vector is a plasmid.

86. The cell of claim 84 wherein said vector is a viral vector.