Electrophoretic apparatus having arrayed electrophoresis lanes

• US 5,192,412 A
• Filed: 11/29/1991
• Issued: 03/09/1993
• Est. Priority Date: 11/30/1990
• Status: Expired due to Term

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Kambara, Hideki, Nagai, Keiichi
• Primary Examiner(s): NIEBLING, JOHN F
• Assistant Examiner(s): Ryser, David G.
• Application Number:
  US07/800,042
• Time in Patent Office: 466 Days
• Field of Search: 204/182.8, 204/299 R
• US Class Current: 204/612
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44782 of a plurality of samples

Abstract

An electrophoretic apparatus includes an electrophoresis panel having a fluted glass plate and a non-fluted plane plate. The fluted glass plate is provided with a plurality of substantially parallel narrow electrophoresis grooves and a groove intersecting the electrophoresis grooves for laser beam irradiation. The fluted glass plate and the non-fluted plane plate are closely superposed on each other to form many capillaries adapted to be filled with gel to thereby form many electrophoresis lanes. The electrophoretic apparatus further includes a light measuring instrument to measure fluorescence images at positions which are subjected to laser beam irradiation. Passage of fluorescently labeled sampled fragments through the laser beam irradiation positions in each electrophoresis lane is detected by measuring a change of fluorescence emission with time.

Claims

1. An electrophoretic apparatus comprising:

an electrophoresis panel including first and second plane plates, a principal surface of said first plane plate being in close contact with said second plane plate and being provided with a plurality of lengthwise grooves which do not intersect one another and a widthwise groove intersecting said lengthwise grooves and extending to opposite edges of said principal surface of said first plane plate, said lengthwise grooves defining gaps adapted to be filled with an electrophoresis medium to provide a plurality of electrophoresis lanes;

voltage applying means for applying an electric field for electrophoresis to said plurality of electrophoresis lanes; and

detection means for discretely detecting fragments passing through the electrophoresis lanes at predetermined detection positions in the electrophoresis panel.

2. An electrophoretic apparatus as recited in claim 1, wherein gel is filled in said gaps defined by said lengthwise grooves.

3. An electrophoretic apparatus as recited in claim 1, wherein said lengthwise grooves extend to opposite edges of the principal surface of the first plane plate.

4. An electrophoretic apparatus as recited in claim 1, wherein said lengthwise grooves are linear and parallel to one another.

5. An electrophoretic apparatus as recited in claim 1, wherein at least one of said first and second plane plates is made of a glass material.

6. An electrophoretic apparatus as recited in claim 1, wherein at least one of said first and second plane plates is a metal plate having at least surface coated with an electrically insulating material.

7. An electrophoretic apparatus as recited in claim 1, wherein a width of each of said lengthwise grooves is not greater than 0.2 mm.

8. An electrophoretic apparatus as recited in claim 7, wherein a cross-sectional area of each of said electrophoresis lanes is not greater than 0.04 mm².

9. An electrophoretic apparatus as recited in claim 1, wherein a number of said lengthwise grooves per 1 cm is at least ten.

10. An electrophoretic apparatus comprising:

an electrophoresis panel including first and second plane plates in close contact with each other, a principal surface of said first plane plate being in close contact with said second plane plate and being provided with a plurality of lengthwise grooves which do not intersect one another and a widthwise groove intersecting said lengthwise grooves and extending to opposite edges of said principal surface of said first plane plate, said lengthwise grooves defining gaps adapted to be filled with an electrophoresis medium to provide electrophoresis lanes in the respective lengthwise grooves;

voltage applying means for applying an electric field for electrophoresis to the plurality of electrophoresis lanes to thereby electrophoretically move fluorescently labeled sample fragments in the electrophoresis lanes;

excitation means for passing at least one light beam from one side of the electrophoresis panel along said widthwise groove for exciting said fluorescently labeled sample fragments to produce fluorescent emissions; and

light detection means for detecting said fluorescent emissions from the sample fragments at said widthwise groove for the respective electrophoresis lanes.

11. An electrophoretic apparatus as recited in claim 10, wherein said light detection means includes position discriminable light detection means disposed to detect said fluorescent emissions along said widthwise groove in a direction substantially perpendicular to an optical path of said at least one light beam extending along said widthwise groove.

12. An electrophoretic apparatus as recited in claim 10, wherein said gaps defined by said lengthwise grooves are filled with gel serving as said electrophoresis medium.

13. An electrophoretic apparatus as recited in claim 10, wherein said lengthwise grooves extend to opposite edges of said principal surface of said first plane plate.

14. An electrophoretic apparatus as recited in claim 10, wherein said lengthwise grooves are linear and parallel to one another.

15. An electrophoretic apparatus as recited in claim 10, wherein at least one of said first and second plane plates is made of a glass material.

16. An electrophoretic apparatus as recited in claim 10, wherein at least one of said first and second plane plates is a metal plate having at least one surface coated with an electrically insulating material.

17. An electrophoretic apparatus as recited in claim 10, wherein a width of each of said lengthwise grooves is not greater than 0.2 mm.

18. An electrophoretic apparatus as recited in claim 17, wherein a cross-sectional area of each of said electrophoresis lanes is not greater than 0.04 mm².

19. An electrophoretic apparatus as recited in claim 10, wherein a number of said lengthwise grooves per 1 cm is at least ten.

20. An electrophoretic apparatus comprising:

an electrophoresis panel including two plates sandwiching therebetween spacers which are arranged in two rows at predetermined intervals in each of said two rows and are in the shape of one of a ribbon and a wire, and an electrophoresis medium filled in spaces defined by said two plates and said spacers to thereby provide a plurality of lengthwise electrophoresis lanes extending between said spacers, and a widthwise lane extending between said two rows in which said spacers are arranged and intersecting said lengthwise electrophoresis lanes;

voltage applying means for applying an electric field for electrophoresis to the plurality of lengthwise electrophoresis lanes; and

detection means for discretely detecting fragments passing through the lengthwise electrophoresis lanes at predetermined detection positions in the electrophoresis panel.

21. An electrophoretic apparatus comprising:

an electrophoresis panel including a first plane plate to which spacers in the form of one of a ribbon and a wire are attached in two rows at predetermined intervals in each of said two rows, and a second plane plate disposed on said spacers, said first and second plane plates and said spacers defining spaces adapted to be filled with an electrophoresis medium to form a plurality of lengthwise electrophoresis lanes extending between said spacers, and a widthwise lane extending between said two rows in which said spacers are arranged and intersecting said lengthwise electrophoresis lanes;

voltage applying means for applying an electric field for electrophoresis to said plurality of lengthwise electrophoresis lanes; and

detection means for discretely detecting fragments passing through said plurality of lengthwise electrophoresis lanes at predetermined detection positions in the electrophoresis panel.