Biosensor utilizing enzyme and a method for producing the same
First Claim
1. A biosensor for quantifying a substrate contained in a sample liquid by reducing electron acceptors using electrons generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically, the biosensor comprising:
- an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode which are formed on the insulating substrate, a reaction layer including the enzyme provided on the electrode system, and a hydrogen ion concentration control layer;
the reaction layer being in contact with the electrode system.
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Abstract
The biosensor of the present invention quantifies a substrate contained in a sample liquid by reducing electron acceptors using electron generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically. The biosensor has an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode, a reaction layer including the enzyme provided on the electrode system and a hydrogen ion concentration control layer, and the reaction layer is in contact with the electrode system. According to the present invention, the hydrogen ion concentration in the sample liquid can be made most appropriate in accordance with the type of the enzyme contained in the reaction layer, without the adjustment of the hydrogen ion concentration in the sample liquid beforehand. Thus, the specific substrate contained in the sample liquid can be easily quantified with accuracy and speed.
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Citations
13 Claims
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1. A biosensor for quantifying a substrate contained in a sample liquid by reducing electron acceptors using electrons generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically, the biosensor comprising:
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an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode which are formed on the insulating substrate, a reaction layer including the enzyme provided on the electrode system, and a hydrogen ion concentration control layer; the reaction layer being in contact with the electrode system. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for producing a biosensor for quantifying a substrate contained in a sample liquid by reducing electron acceptors using electrons generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically, the method comprising the steps of:
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firstly, providing an electrode system including at least a working electrode and a counter electrode on an electrical insulating substrate; secondly, forming a hydrogen ion concentration control layer including electron acceptors on the insulating substrate; and finally, forming a reaction layer including hydrophilic polymer and enzyme on the electrode system.
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Specification