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Biosensor utilizing enzyme and a method for producing the same

  • US 5,192,415 A
  • Filed: 03/02/1992
  • Issued: 03/09/1993
  • Est. Priority Date: 03/04/1991
  • Status: Expired due to Term
First Claim
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1. A biosensor for quantifying a substrate contained in a sample liquid by reducing electron acceptors using electrons generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically, the biosensor comprising:

  • an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode which are formed on the insulating substrate, a reaction layer including the enzyme provided on the electrode system, and a hydrogen ion concentration control layer;

    the reaction layer being in contact with the electrode system.

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