Method and apparatus for batch injection analysis

• US 5,192,416 A
• Filed: 04/09/1991
• Issued: 03/09/1993
• Est. Priority Date: 04/09/1991
• Status: Expired due to Fees

• Current Assignee: New Mexico State University Technology Transfer Corporation
• Original Assignee: New Mexico State University Technology Transfer Corporation
• Inventors: Taha, Ziad H., Wang, Joseph
• Primary Examiner(s): Niebling, John
• Assistant Examiner(s): BELL, BRUCE F
• Application Number:
  US07/682,907
• Time in Patent Office: 700 Days
• Field of Search: 436/52, 436/53, 204/409, 204/412, 204/416, 204/433, 204/153.21, 204/153.1, 204/400
• US Class Current: 204/409
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00

Abstract

Batch injection analysis comprises apparatus and method for injecting and transporting analytes toward a detector immersed in a confined, inert electrolyte. Passage of the analyte over the detector surface provides measurement of sample concentrations. Detectors normally comprise selective electrodes, such as biologically or chemically modified surfaces, ion-selective probes, optical or thermal devices, thus eliminating conduits, valves, and pumps.

Claims

1. A batch injection analysis apparatus for measuring analyte sample concentration comprising:

vessel means;

single batch electrolyte means confined within said vessel means;

means for serially introducing into said vessel means, for dilution in said single batch electrolyte means, multiple analyte samples to be analyzed, the volume of each said analyte sample having a proportional ratio to the volume of said single batch electrolyte means within the range of 1/20,000 to 1/50,000;

planar detection means in said vessel means for sensing said analyte samples to be analyzed; and

means for analyzing said analyte samples.

2. The invention of claim 1 wherein said vessel means further comprises stirrer means.

3. The invention of claim 2 wherein said stirrer means comprises magnetic stirrer means.

4. The invention of claim 1 wherein said electrolyte means is inert relative to said analyte sample.

5. The invention of claim 1 wherein said electrolyte means comprises at least one solution selected from the group consisting of potassium chloride, a phosphate buffer and sodium hydroxide, potassium dihydrogen phosphate and sodium nitrate, and CH₃ COONa, CH₃ COOH, NaC1, and 1,2 diaminocyclohexane-N,N,N'"'"',N'"'"', tetraacetic acid.

6. The invention of claim 5 wherein said electrolyte means comprises CH₃ COONa, CH₃ COOH, NaC1;

1,2 diaminocyclohexane-N,N,N'"'"',N'"'"', tetraacetic acid; and

aluminum sulfate.

7. The invention of claim 1 wherein said means for introducing analyte samples into said vessel means comprises injection means.

8. The invention of claim 7 wherein said injection means comprises pipette means.

9. The invention of claim 1 wherein said detector means comprises electrode means.

10. The invention of claim 9 wherein said electrode means comprises carbon paste.

11. The invention of claim 10 wherein said carbon paste electrode means further comprises at least one member selected from the group consisting of glucose oxidase and ruthenium dioxide.

12. The invention of claim 9 wherein said electrode means comprises pH electrode means.

13. The invention of claim 9 wherein said electrode means comprises ion-selective electrode means.

14. The invention of claim 13 wherein said ion-selective electrode means comprises a member selected from the group consisting of chloride electrode means and fluoride electrode means.

15. The invention of claim 1 wherein said means for analyzing said analyte samples comprises amperometric means.

16. The invention of claim 1 wherein said means for analyzing said analyte samples comprises potentiometric means.

17. The invention of claim 1 wherein said means for introducing samples to be analyzed and said means for sensing said samples are positioned at a distance from each other less than 2 mm.

18. The invention of claim 1 wherein said means for introducing samples to be analyzed and said means for sensing said samples are positioned at a distance from each other within 2 to 10 mm.

19. The invention of claim 1 wherein said means for introducing samples to be analyzed and said means for sensing said samples are positioned at a distance from each other greater than 10 mm.

20. A batch injection analytical method for measuring analyte sample concentration comprising the steps of:

a) providing a vessel;

b) confining a single batch of an electrolyte within the vessel;

c) serially introducing into the vessel, for dilution in the single batch of electrolyte, multiple samples to be analyzed, such that the ratio of each sample volume to the single batch of electrolyte volume is within the range of 1/20,000 to 1/50,000;

d) sensing the samples to be analyzed; and

e) analyzing the samples.

21. The method of claim 20 further comprising the step of stirring the samples.

22. The method of claim 20 wherein the step of confining an electrolyte within the vessel comprises the step of confining an inert electrolyte within the vessel.

23. The method of claim 22 wherein the step of confining an inert electrolyte within the vessel comprises the step of confining at least one solution selected from the group consisting of potassium chloride;

a phosphate buffer and sodium hydroxide;

potassium dihydrogen phosphate and sodium nitrate; and

CH₃ COONa, CH₃ COOH, NaC1, and 1,2 diaminocyclohexane-N,N,N'"'"',N'"'"', tetraacetic acid.

24. The method of claim 23 wherein the step of confining an inert electrolyte within the vessel comprises the step of confining CH₃ COONa, CH₃ COOH, NaC1;

1,2 diaminocyclohexane-N,N,N'"'"',N'"'"', tetraacetic acid; and

aluminum sulfate.

25. The method of claim 20 wherein the step of introducing samples to be analyzed into the vessel comprises the step of injecting analyte samples into the vessel.

26. The method of claim 25 wherein the step of injecting samples into the vessel further comprises pipetting analyte samples into the vessel.

27. The method of claim 20 wherein the step of sensing the analyte samples to be analyzed comprises the step of detecting the samples with an electrode.

28. The method of claim 27 wherein the step of detecting the analyte samples with an electrode comprises providing a carbon paste electrode.

29. The method of claim 28 wherein the step of providing a carbon paste electrode further comprises the step of providing glucose oxidase on the electrode.

30. The method of claim 28 wherein the step of providing a carbon paste electrode further comprises the step of providing ruthenium dioxide on the electrode.

31. The method of claim 27 wherein the step of detecting the samples with an electrode comprises providing a pH electrode.

32. The method of claim 27 wherein the step of detecting the samples with an electrode comprises providing an ion-selective electrode.

33. The method of claim 32 wherein the step of providing ion-selective electrodes comprises providing a member selected from the group consisting of chloride electrodes and fluoride electrodes.

34. The method of claim 20 wherein the step of analyzing the analyte samples comprises measuring amperometric responses of the analyte samples.

35. The method of claim 20 wherein the step of analyzing the analyte samples comprises measuring potentiometric responses of the analyte samples.

36. The method of claim 20 further comprising the step of positioning the means for introducing samples and the sensing means at a distance under 2 mm from each other.

37. The method of claim 20 further comprising the step of positioning the means for introducing samples and the sensing means at a distance within 2 mm to 10 mm from each other.

38. The method of claim 20 further comprising the step of positioning the means for introducing samples and the sensing means at a distance greater than 10 mm from each other.