Receptor-based biosensors
First Claim
1. An opiate receptor-containing biosensor for the determination of an opiate in a sample comprising:
- a) a polymeric film formed by chemical copolymerization of a solution comprising;
1) a base component which is a protein,2) an opiate receptor; and
3) at least one stabilizer selected from the group consisting of lipids, detergents and antioxidants;
b) a first sensor comprising a first conductor and a second conductor which are in contact with said film; and
c) a current source in conductive contact with the first and second conductors for measuring an electrical characteristic of the polymeric film.
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Accused Products
Abstract
A method of immobilizing and stabilizing an active biological receptor in a polymeric film and receptor-based biosensors for determining an analyte of interest in a sample. The receptor-based biosensors include a polymeric film having a biological receptor capable of binding an analyte of interest immobilized therein according to the method of the present invention and an electrical means for determining the presence and quantity of the analyte. In particular, acetylcholine receptor and opiate receptor have been immobilized in a polymeric film made by combining the receptor, a material (e.g., bovine serum albumin, gelatin) capable of polymerizing and a polymerizing agent (e.g., glutaradehyde).
251 Citations
9 Claims
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1. An opiate receptor-containing biosensor for the determination of an opiate in a sample comprising:
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a) a polymeric film formed by chemical copolymerization of a solution comprising; 1) a base component which is a protein, 2) an opiate receptor; and 3) at least one stabilizer selected from the group consisting of lipids, detergents and antioxidants; b) a first sensor comprising a first conductor and a second conductor which are in contact with said film; and c) a current source in conductive contact with the first and second conductors for measuring an electrical characteristic of the polymeric film. - View Dependent Claims (2, 3, 5)
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4. A double chip, opiate receptor-containing biosensor for the determination of an opiate in a sample, comprising a first polymeric film comprising a protein, a stabilizer and an opiate receptor, the first polymeric film being in contact with a first electrical sensor for measuring an electrical characteristic of the first polymeric film and further comprising:
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a) a second nonreceptor containing polymeric film, the second polymeric film being in contact with a second electrical sensor for measuring an electrical characteristic of the second polymeric film; b) a potential barrier interposed between the second nonreceptor containing film and the first polymeric film, such that the barrier hinders current flow between said second nonreceptor containing film and the first polymeric film; c) a current source; and d) a circuit contacting the current source and the first and the second electrical sensors for measuring the absolute and the differential changes in the electrical characteristics of the first and second films. - View Dependent Claims (6)
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7. A method of immobilizing and stabilizing an opiate receptor in a polymeric film on a transducer, comprising:
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a. forming a mixture by combining an opiate receptor, a base component which is a protein, a polymerizing agent and at least one stabilizer selected from the group consisting of lipids, detergents and antioxidants, under conditions appropriate for polymerization to occur; and b. forming a film by polymerization of the mixture of a) on the transducer. - View Dependent Claims (8)
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9. A method for determining the presence of an opiate in a sample comprising:
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a) contacting the sample with a biosensor comprising; 1) a polymeric film formed by chemical copolymerization of a solution comprising; a) a base component which is a protein; b) an opiate receptor capable of binding the analyte; and c) at least one stabilizer selected from the group consisting of lipids, detergents and antioxidants; and 2) a sensor comprising a first conductor and a second conductor which are in contact with the polymeric protein film; and b) determining a change in the electrical characteristic of the polymeric protein film, whereby a change is indicative of the presence of analyte in the sample.
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Specification