Isolation and preservation of fetal and neonatal hematopoietic stem and progenitor cells of the blood and methods of therapeutic use
DCFirst Claim
1. A method for obtaining human neonatal for fetal hematopoietic stem or progenitor cells comprising:
- (a) isolating human neonatal or fetal blood components containing hematopoietic stem or progenitor cells;
(b) cryopreserving the blood components; and
(c) thawing the blood components,such that the stem or progenitor cells are viable.
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Abstract
The present invention relates to hematopoietic stem and progenitor cells of neonatal or fetal blood that are cryopreserved, and the therapeutic uses of such stem and progenitor cells upon thawing. In particular, the present invention relates to the therapeutic use of fetal or neonatal stem cells for hematopoietic (or immune) reconstitution. Hematopoietic reconstitution with the cells of the invention can be valuable in the treatment or prevention of various diseases and disorders such as anemias, malignancies, autoimmune disorders, and various immune dysfunctions and deficiencies. In another embodiment, fetal or neonatal hematopoietic stem and progenitor cells which contain a heterologous gene sequence can be used for hematopoietic reconstitution in gene therapy. In a preferred embodiment of he invention, neonatal or fetal blood cells that have been cryopreserved and thawed can be used for utologous (self) reconstitution.
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Citations
64 Claims
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1. A method for obtaining human neonatal for fetal hematopoietic stem or progenitor cells comprising:
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(a) isolating human neonatal or fetal blood components containing hematopoietic stem or progenitor cells; (b) cryopreserving the blood components; and (c) thawing the blood components, such that the stem or progenitor cells are viable. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for hematopoietic or immune reconstitution of a human comprising:
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(a) isolating human neonatal or fetal blood components containing hematopoietic stem cells; (b) cryopreserving the blood components; (c) thawing the blood components; and (d) introducing the blood components into a suitable human host, such that the hematopoietic stem cells are viable and can proliferate within the host. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
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47. A method for hematopoietic or immune reconstitution of a human comprising:
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(a) isolating human neonatal or fetal blood components containing hematopoietic stem and progenitor cells; (b) cryopreserving the blood components; (c) thawing the blood components; and (d) introducing the blood components into a suitable human host, such that the hematopoietic stem and progenitor cells are viable and can proliferate within the host. - View Dependent Claims (48, 49, 50, 51, 52, 53, 54)
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55. A method for obtaining cryopreserved human neonatal or fetal hematopoietic stem cells derived from the blood comprising:
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(a) isolating human neonatal or fetal blood components containing human neonatal or fetal hematopoietic stem cells; and (b) cryopreserving the blood components, such that the stem cells remain viable. - View Dependent Claims (56)
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- 57. A method for hematopoietic or immune reconstitution of a human comprising introducing into the human a composition comprising human neonatal or fetal hematopoietic stem cells derived from the blood, in which the stem cells have been previously cryopreserved.
- 60. A method for hematopoietic or immune reconstitution of a human comprising introducing into the human a composition comprising human neonatal or fetal hematopoietic stem cells derived from the blood, in which the stem cells are progeny of cells which have been previously cryopreserved.
Specification