Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes

• US 5,192,659 A
• Filed: 07/11/1990
• Issued: 03/09/1993
• Est. Priority Date: 08/25/1989
• Status: Expired due to Term

• Current Assignee: Genetic Technologies Limited
• Original Assignee: Genetype AG (Genetic Technologies Limited)
• Inventors: Simons, Malcolm J.
• Primary Examiner(s): Moskowitz, Margaret
• Assistant Examiner(s): YUAN, LORI
• Application Number:
  US07/551,239
• Time in Patent Office: 972 Days
• Field of Search: 435/6, 435/91, 435/810, 436/63, 436/94, 436/501, 436/808, 935/76, 935/77, 935/78, 935/17, 935/18
• US Class Current: 435/6.11
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 1/683 involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
• CPC Class Codes: C12Q 1/6858 Allele-specific amplification
• CPC Class Codes: C12Q 1/6881 for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2521/307 Single strand endonuclease
• CPC Class Codes: C12Q 2535/125 Allele specific primer extension
• CPC Class Codes: C12Q 2539/105 Involving introns, exons, or splice junctions
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/172 Haplotypes

Abstract

The present invention provides a method for detection of at least one allele of a genetic locus and can be used to provide direct determination of the haplotype. The method comprises amplifying genomic DNA with a primer pair that spans an intron sequence and defines a DNA sequence in genetic linkage with an allele to be detected. The primer-defined DNA sequence contains a sufficient number of intron sequence nucleotides to characterize the allele. Genomic DNA is amplified to produce an amplified DNA sequence characteristic of the allele. The amplified DNA sequence is analyzed to detect the presence of a genetic variation in the amplified DNA sequence such as a change in the length of the sequence, gain or loss of a restriction site or substitution of a nucleotide. The variation is characteristic of the allele to be detected and can be used to detect remote alleles. Kits comprising one or more of the reagents used in the method are also described.

Claims

1. A method for detection of at least one coding region allele of an HLA locus comprising amplifying genomic DNA with a primer pair that spans a non-coding region sequence selected from the group consisting of untranslated sequences between exons, 5'"'"' and 3'"'"' untranslated regions associated with a genetic locus, and spacing sequences between genetic loci, said primer pair defining a DNA sequence, said DNA sequence being in genetic linkage with said HLA locus and containing a sufficient number of said non-coding region sequence nucleotides to produce an amplified DNA sequence characteristic of said allele.

2. The method of claim 1 wherein said amplified DNA sequence includes at least about 300 nucleotides corresponding to intron sequences.

3. The method of claim 1 wherein said intron sequence is adjacent to an exon encoding said allele.

4. The method of claim 1 wherein said amplified DNA sequence is characteristic of at least one nonadjacent allele.

5. The method of claim 1 wherein said amplified DNA sequence is characteristic of at least one adjacent allele and at least one nonadjacent allele.

6. The method of claim 5 wherein said amplified DNA sequence includes at least about 1,000 nucleotides corresponding to non-coding region sequences.

7. A method for detection of at least one allele of an HLA locus comprising:

A. amplifying genomic DNA with a primer pair that spans a non-coding region sequence selected from the group consisting of untranslated sequences between exons, 5'"'"' and 3'"'"' untranslated regions associated with a genetic locus, and spacing sequences between genetic loci, said primer pair defining a DNA sequence, said DNA sequence being in genetic linkage with said allele and containing a sufficient number of said non-coding region sequence nucleotides to produce an amplified DNA sequence characteristic of said allele; and

B. analyzing said amplified DNA sequence to detect the presence of a genetic variation in said amplified sequence.

8. The method of claim 7 wherein said variation in said amplified DNA sequence is a variation in the length of the primer-defined amplified DNA sequence.

9. The method of claim 7 wherein said variation in said amplified DNA sequence is a change in the presence of at least one restriction site in the primer-defined amplified DNA sequence.

10. The method of claim 7 wherein said variation in said amplified DNA sequence is a change in the location of at least one restriction site in the primer-defined amplified DNA sequence.

11. The method of claim 7 wherein said variation in said amplified DNA sequence is a substitution of at least one nucleotide in the primer-defined amplified DNA sequence.