Mutant strain of C. acetobutylicum and process for making butanol
First Claim
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1. A method of producing butanol which comprises anaerobically culturing a biologically pure culture of Clostridium acetobutylicum ATCC 55025 in a nutrient medium with assimilable sources of carbon, nitrogen, and inorganic substances and then recovering the butanol.
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Abstract
A biologically pure asporogenic mutant of Clostridium acetobutylicum is produced by growing sporogenic C. acetobutylicum ATCC 4259 and treating the parent strain with ethane methane sulfonate. The mutant which as been designated C. acetobutylicum ATCC 55025 is useful in an improved ABE fermentation process, and produces high concentrations of butanol and total solvents.
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Citations
2 Claims
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1. A method of producing butanol which comprises anaerobically culturing a biologically pure culture of Clostridium acetobutylicum ATCC 55025 in a nutrient medium with assimilable sources of carbon, nitrogen, and inorganic substances and then recovering the butanol.
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2. A biologically pure culture of clostridium acetobutylicum ATCC 55025.
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Current AssigneeThe Michigan Biotechnology Institute
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Original AssigneeThe Michigan Biotechnology Institute
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InventorsBeacom, Daniel, Datta, Rathin, Jain, Mahendra K.
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Primary Examiner(s)Naff, David M.
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Assistant Examiner(s)Knode, Marian C.
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Application NumberUS07/516,610Time in Patent Office1,044 DaysField of Search435/160, 435/252.7, 435/842US Class Current435/160CPC Class CodesC12N 1/205 Bacterial isolatesC12R 2001/145 ClostridiumY10S 435/842 Clostridium
