Growing Ehrlichia species in a continuous cell line
First Claim
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1. A method for continually growing a bacterial pathogen comprising infecting the immortal, canine, macrophage cell line DH82 with Ehrlichia canis and cultivating the infected cells in a suitable culture medium until at least 30% of the DH82 cells are infected.
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Abstract
The present invention relates to a method of continually propagating Ehrlichia canis in a microphage-monocyte cell line referred to as DH82.
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5 Claims
- 1. A method for continually growing a bacterial pathogen comprising infecting the immortal, canine, macrophage cell line DH82 with Ehrlichia canis and cultivating the infected cells in a suitable culture medium until at least 30% of the DH82 cells are infected.
- 3. A viable DH82 cell infected with Ehrlichia canis.
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