Growing Ehrlichia species in a continuous cell line

• US 5,192,679 A
• Filed: 05/03/1990
• Issued: 03/09/1993
• Est. Priority Date: 05/03/1990
• Status: Expired due to Term

• Current Assignee: United States Department Of Health And Human Services (Government of the United States of America), The Ohio State University Research Foundation
• Original Assignee: United States Department Of Health And Human Services (Government of the United States of America)
• Inventors: Rikihisa, Yasuko, Dawson, Jacqueline E.
• Primary Examiner(s): Robinson, Douglas W.
• Assistant Examiner(s): WILLIAMS, JANE
• Application Number:
  US07/518,182
• Time in Patent Office: 1,041 Days
• Field of Search: 424/88, 424/93, 435/235, 435/240.3, 435/240.21, 435/240.23, 435/253, 435/260, 435/258, 435/245
• US Class Current: 435/243
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor

Abstract

The present invention relates to a method of continually propagating Ehrlichia canis in a microphage-monocyte cell line referred to as DH82.

Claims

1. A method for continually growing a bacterial pathogen comprising infecting the immortal, canine, macrophage cell line DH82 with Ehrlichia canis and cultivating the infected cells in a suitable culture medium until at least 30% of the DH82 cells are infected.

2. The method of claim 1 wherein at least 60% of the DH82 cells are infected.

3. A viable DH82 cell infected with Ehrlichia canis.

4. A culture of cells according to claim 3 in which at least 30% of said cells are infected.

5. A culture of cells according to claim 4 in which at least 60% of said cells are infected.